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    • 40. 发明专利
    • A METHOD AND EQUIPMENT FOR CRYOPRESERVATION OF GARLIC CALLUS
    • AU2021101536A4
    • 2021-05-13
    • AU2021101536
    • 2021-03-25
    • INST VEGETABLES & FLOWERS CAAS
    • WANG HAIPINGLIU ZHUOYALI XIXIANGSONG JIANGPINGZHAO QINGZHANG XIAOHUI
    • A23B7/055A01H4/00A01H6/04C12M1/22C12M3/00C12N5/00
    • The application belongs to the technical field of cryopreservation of agricultural products, it discloses a method and an equipment for cryopreservation of garlic callus. Taking the callus that grows well and pre-incubate; Using cryoprotectant for loading, dehydration and vitrification; Rewarming of the callus after cryoprotectant treatment; Recovery of the callus after cryoprotectant treatment; And connecting the recovered callus to the culture medium for primary culture and propagation. Through the method of the application, the effect of cryopreservation of garlic tissue and the survival rate of garlic callus after cryopreservation are improved: The pre-cultivation time is 2 days, the time of media loading is 20 minutes, and the PVS2 vitrification solution treatment is 15 minutes, which will significantly increase the survival rate of callus. The method of the application can be applied to cryopreservation of garlic callus, its operation process is simple, easy to be applied to production, and can be used for efficient and safe preservation of garlic germplasm resources. Callus with good growth is inserted into petri dishes containing Gamborg B5 basic medium Si0l +NAA+21P, and the inoculated petri dishes are placed in the dark for pre-incubate; Using PVS2 as a cryoprotectant for loading, dehydration and vitrification; -S102 Rewarming ofcallus after PVS2 treatment; 'S103 Recovery ofthe callus after PVS2 treatment