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    • 45. 发明申请
    • IN VITRO ASSAY FOR IDENTIFICATION OF ALLERGENIC PROTEINS
    • 用于鉴定过敏蛋白的体外测定
    • US20090098545A1
    • 2009-04-16
    • US12067042
    • 2006-09-14
    • Karin CederbrantHanna Lundgren
    • Karin CederbrantHanna Lundgren
    • C12Q1/68C12Q1/02
    • G01N33/6803C07K14/825C12Q1/6883C12Q2600/158G01N33/5008G01N33/5023G01N33/5047G01N33/5094
    • The present invention relates to a process for in vitro evaluation, of a potentially allergenic or tissue irritating sub-stance whereby test cells are cultivated in the presence of the substance, and the presence of up regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, SPR, GNB2, XK, IFITM3, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MT1G, MT1B; MT1A, ADFP, JL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also regards use of the expression products from one or more of the genes for in vitro analysis of allergy or tissue irritation. It also relates to a probe comprising at least three nucleic acids, preferably 3-40, especially 5-15 chosen from RNA complementary to the RNA corresponding to any of the genes and the use thereof for in vitro analysis of allergy or tissue irritation. Further it regards a reagent kit comprising one or more probes that recognize products produced during the expression of any the genes.
    • 本发明涉及一种用于体外评估潜在致敏原或组织刺激性的方法,其中在物质存在下培养测试细胞,并且存在选自G1P2,OASL,IFIT1,TRIM22的上调基因 ,IFI44L,MXI,RSAD2,IFIT3,IFITM1,IFIT2,SPR,GNB2,XK,IFITM3,C33.28 HERV-H蛋白mRNA,IFITM3,XK,GPR15,MT1G,MT1B; 测量MT1A,ADFP,JL8,MT1E,MT1F,MT1H,SLC30A1,SERPINB2,CD83,TncRNA或其表达产物。 本发明还涉及来自一种或多种基因的表达产物用于过敏或组织刺激的体外分析。 它还涉及包含至少三种核酸,优选3-40,特别是5-15的探针,其选自与对应于任何基因的RNA互补的RNA及其用于过敏或组织刺激的体外分析的用途。 此外,它涉及一种试剂盒,其包含一种或多种识别在任何基因表达过程中产生的产物的探针。