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    • 75. 发明申请
    • METHOD FOR AMPLIFYING DNA FRAGMENT
    • 放大DNA片段的方法
    • US20100120036A1
    • 2010-05-13
    • US12530033
    • 2008-03-05
    • Kunio ShiotaShintaro YagiYoko TakahashiSatoshi TanakaJun Ohgane
    • Kunio ShiotaShintaro YagiYoko TakahashiSatoshi TanakaJun Ohgane
    • C12Q1/68C12P19/34
    • C12Q1/683C12Q2531/113C12Q2525/155C12Q2521/331
    • To measure the methylation degree of a genomic DNA simply and correctly as well as exhaustively, there is provided a method for amplifying a DNA fragment, characterized by comprising the following steps (1) to (5): (1) digesting a sample double-stranded DNA with a first restriction enzyme; (2) adding a double-stranded DNA fragment consisting of a first oligonucleotide and a second oligonucleotide to a restriction enzyme cleavage end of the double-stranded DNA fragment produced in step (1) to obtain a DNA fragment; (3) digesting the DNA fragment obtained in step (2) with a second restriction enzyme; (4) allowing the double-stranded DNA fragment produced in step (3) to coexist with a third oligonucleotide and a fourth oligonucleotide in the presence of a ligase; and (5) heating the double-stranded DNA fragment obtained in step (4), thereby dissociating the double-stranded DNA fragment and then performing a polymerase chain reaction to selectively amplify a DNA fragment having the cleavage end of the first restriction enzyme and the cleavage end of the second restriction enzyme.
    • 为了简单正确地,全面地测定基因组DNA的甲基化程度,提供了扩增DNA片段的方法,其特征在于包括以下步骤(1)〜(5):(1) 用第一限制酶的双链DNA; (2)将由第一寡核苷酸和第二寡核苷酸组成的双链DNA片段加入步骤(1)中制备的双链DNA片段的限制酶切割末端以获得DNA片段; (3)用第二限制酶消化步骤(2)中获得的DNA片段; (4)在连接酶存在下使步骤(3)中产生的双链DNA片段与第三寡核苷酸和第四寡核苷酸共存; (5)加热步骤(4)中获得的双链DNA片段,从而解离双链DNA片段,然后进行聚合酶链式反应以选择性扩增具有第一限制酶切割末端的DNA片段, 第二限制酶的切割末端。
    • 78. 发明申请
    • INTAKE AIR HEATING CONTROL DEVICE FOR GAS TURBINE
    • 采用气体涡轮机的空气加热控制装置
    • US20100071372A1
    • 2010-03-25
    • US12447382
    • 2007-11-09
    • Satoshi TanakaShoichi HaradaKozo Toyama
    • Satoshi TanakaShoichi HaradaKozo Toyama
    • F02C1/00F28F27/00
    • F02C9/22F02C7/047F02C7/057F05D2270/705
    • Extremely cold (e.g., −20° C.) air A is heated by a heat exchanger 30, which is supplied with steam S via a control valve 32, and heated air A′ is taken into a gas turbine 10. The valve opening degree of the control valve 32 is feedback-controlled so that the deviation between the measured temperature t1 and the target temperature TO of the heated air A′ is eliminated. Further, when the number of revolutions, N, of the gas turbine 10 increases, or when an IGV opening degree OP increases, the valve opening degree of the control valve 32 is feedforward-controlled in accordance with the increase in the number N of revolutions or the increase in the IGV opening degree OP. By so doing, the temperature of air A′ can be maintained at a temperature enabling stable combustion without delay in control, and intake air can be heated without delay in control, even at the start of the gas turbine or during change in the opening degree of an inlet guide vane.
    • 非常冷(例如-20℃),空气A被热交换器30加热,热交换器30经由控制阀32供给蒸汽S,加热空气A'被吸入燃气轮机10.阀开度 控制阀32被反馈控​​制,从而消除了加热空气A'的测量温度t1与目标温度TO之间的偏差。 此外,当燃气轮机10的转数N增加时,或当IGV开度OP增加时,控制阀32的阀开度根据转数N的增加进行前馈控制 或IGV开度OP的增加。 通过这样做,空气A'的温度可以保持在能够在不延迟控制的情况下稳定燃烧的温度,并且即使在燃气轮机的启动时或者在开度变化的情况下,也可以不延迟地加热进气 的入口导叶。
    • 79. 发明授权
    • Standard cell, cell library using a standard cell and method for arranging via contact
    • 标准细胞,使用标准细胞的细胞库和通过接触排列的方法
    • US07681162B2
    • 2010-03-16
    • US11616103
    • 2006-12-26
    • Satoshi Tanaka
    • Satoshi Tanaka
    • G06F17/50
    • H01L27/0207G06F17/5072H01L27/11807
    • According to an aspect of the present invention, there is provided a standard cell, including a cell frame having a rectangular region, a power supply interconnection, a center line of the power supply interconnection overlapping with a side line along a first direction of the cell frame, a via contact, the center of the via contact overlapping with the center line of the power supply interconnection and a grid having a minimum interval in a layout of the via contacts, wherein a width of the cell along the first direction is an integral multiplication of the grid and the center of the via contact is at a distance of at least 1.5 multiplication of the grid from the nearest apex in the cell frame.
    • 根据本发明的一个方面,提供了一种标准单元,包括具有矩形区域的单元框架,电源互连,电源互连的中心线沿着电池单元的第一方向与侧线重叠 框架,通孔触点,通孔触点的中心与电源互连的中心线重叠,并且具有在通孔触点的布局中具有最小间隔的格栅,其中沿着第一方向的单元的宽度是积分的 栅格和通孔接触点的中心的乘法距离格栅与单元格框架中最近的顶点的至少1.5倍的距离。