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    • 93. 发明授权
    • Anti-microbial sensitivity test and testing stratum
    • 抗菌敏感性试验和试验层
    • US4820292A
    • 1989-04-11
    • US199379
    • 1988-05-27
    • Bernard KorolPaul Nathan
    • Bernard KorolPaul Nathan
    • A61L15/24A61L15/44A61L26/00C08K5/41C08K5/10C08K5/15C08K5/34
    • A61L26/008A61L15/24A61L15/44A61L26/0014A61L2300/102A61L2300/104A61L2300/204A61L2300/404
    • An anti-microbial sensitivity test and testing stratum is disclosed for evaluating the sensitivity of different anti-microbial agents relative to identifiable microbial contaminants. The test stratum comprises a polymer such as poly(2-hydroxyethylmethacrylate) referred to as PHEMA, an organic solvent, such as polyethylene glycol (PEG), a hydrogen binding plasticizer, such as dimethylsulfoxide (DMSO), an an anti-microbial agent such as silver sulfadiazine, mafenide, nystatin, nitrofurazone, silver nitrate, bacitracin, gentamicin, amphotericin B, cesium nitrate, or other anti-microbial agents. The test includes positioning testing stratum of the type described, each having a different anti-microbial agent therein, on a sterile plate or on an agar substrate and spaced in fixed relationship to one another. The testing stratum are self adhering and remain fixed on a sterile plate, or on an applicator, even when the sterile plate, is inverted. The testing stratum further provide a sustained release of the anti-microbial agent incorporated therein. The testing stratum are then subjected to samples from the wound site, often containing microbial contaminants, and after a predetermined period of incubation, evaluation indicative of microbial contamination can then be made and examination of the zones of inhibition over and around each of the anti-microbial testing stratum will determine effectiveness of each anti-microbial agent contained in the test system.
    • 公开了抗微生物敏感性测试和测试层,用于评估不同抗微生物剂相对于可识别的微生物污染物的敏感性。 测试层包括聚合物,例如称为PHEMA的聚(2-羟乙基甲基丙烯酸酯),有机溶剂如聚乙二醇(PEG),氢结合增塑剂如二甲基亚砜(DMSO),抗微生物剂如 作为磺胺嘧啶银,马法尼酸,制霉菌素,硝基呋喃西林,硝酸银,杆菌肽,庆大霉素,两性霉素B,硝酸铯或其他抗微生物剂。 该测试包括所述类型的定位测试层,每层具有不同的抗微生物剂,在无菌板或琼脂基底上,彼此间隔固定。 测试层是自粘的,并保持固定在无菌板上或涂抹器上,即使无菌板倒置。 测试层进一步提供其中并入的抗微生物剂的持续释放。 然后将测试层从伤口部位进行样品,通常含有微生物污染物,并且在预定的温育期之后,然后可以进行指示微生物污染的评估,并检查每个抗 - 微生物测试层将确定测试系统中所含的每种抗微生物剂的有效性。