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21. 发明授权

US10059960B2 AXMI221z, AXMI222z, AXMI223z, AXMI224z, and AXMI225z delta-endotoxin genes and methods for their use 有权
公开(公告)号：US10059960B2
公开(公告)日：2018-08-28
申请号：US14932494
申请日：2015-11-04
申请人： Athenix Corp.
发明人： Kimberly Sampson , Daniel Tomso
IPC分类号： C12N15/82 , C07K14/325 , A01N37/46 , A01N63/02 , C07K16/12
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , C07K16/1278 , C12N15/8279 , Y02A40/162
摘要： Compositions and methods for conferring lepidoptericidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for the Axmi222z toxin polypeptide are provided. The Axmi222z coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated Axmi222z toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the Axmi222z polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:24-26, or the nucleotide sequence set forth in SEQ ID NO:2, 7, 12, and 17, as well as variants and fragments thereof.

22. 发明授权

US09982274B2 AXMI115 variant insecticidal gene and methods for its use 有权
公开(公告)号：US09982274B2
公开(公告)日：2018-05-29
申请号：US14851555
申请日：2015-09-11
申请人： ATHENIX CORP.
发明人： Nalini Manoj Desai , Volker Heinrichs , Duane Lehtinen
IPC分类号： C12N15/82 , C07K14/325 , A01N63/02 , C07K14/32
CPC分类号： C12N15/8286 , A01N37/44 , A01N63/02 , C07K14/32 , C07K14/325 , C07K2319/00 , C07K2319/24 , C12N15/8279 , C12N15/8285 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2, 3, 7-14 and 47, including biologically active variants and fragments thereof.

23. 发明授权

US09909140B2 AXMI-115, AXMI-113, AXMI-005, AXMI-163 and AXMI-184: insecticidal proteins and methods for their use 有权
公开(公告)号：US09909140B2
公开(公告)日：2018-03-06
申请号：US14733552
申请日：2015-06-08
申请人： Athenix Corporation
发明人： Kimberly S. Sampson , Daniel J. Tomso , Shruti Agarwal , Brian McNulty , Chris Campbell
IPC分类号： C12N15/82 , C07K14/325 , C07K14/32 , A01N37/18 , C07K16/12
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.

24. 发明授权

US09862967B2 AXMI281 toxin gene and methods for its use 有权
公开(公告)号：US09862967B2
公开(公告)日：2018-01-09
申请号：US14910342
申请日：2014-08-08
申请人： Athenix Corp.
发明人： Rebecca Thayer , Kimberly S. Sampson , Duane Lehtinen , Cheryl Peters
IPC分类号： C12N15/82 , C07K14/325 , A01N63/02
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-5, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.

25. 发明授权

US09528122B2 Axmi270 toxin gene and methods of use 有权
标题翻译： Axmi270毒素基因及使用方法
公开(公告)号：US09528122B2
公开(公告)日：2016-12-27
申请号：US14233431
申请日：2012-07-27
申请人： Kimberly S Sampson , Duane A Lehtinen
发明人： Kimberly S Sampson , Duane A Lehtinen
IPC分类号： C12N15/82 , C07K14/325 , A01N63/02
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-5, or the nucleotide sequence set forth in SEQ ID NO:2, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3-5所示的氨基酸序列的核苷酸序列或SEQ ID NO：2所示的核苷酸序列，以及其变体和片段。

26. 发明授权

US09238823B2 Pesticidal genes from brevibacillus and methods for their use 有权
标题翻译：来自短纤维杆菌的杀虫基因及其使用方法
公开(公告)号：US09238823B2
公开(公告)日：2016-01-19
申请号：US13779524
申请日：2013-02-27
申请人： Athenix Corporation
发明人： Kimberly S. Sampson , Daniel J. Tomso , Rong Guo
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C07K14/345 , C12N15/32 , A01N37/18 , A01N25/26 , C07K14/195 , C07K14/325
CPC分类号： C12N15/8286 , C07K14/195 , C07K14/325 , C07K14/345 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含短杆菌属来源的δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了具有编码SEQ ID NO：2,4,7或10所示的氨基酸序列的核苷酸序列的分离的核酸分子或者SEQ ID NO： 5，6，8，9，11，12，13，14或15，以及其变体和片段。

27. 发明申请

US20140223599A1 AXMI279 PESTICIDAL GENE AND METHODS FOR ITS USE 审中-公开
标题翻译： AXMI279农药基因及其使用方法
公开(公告)号：US20140223599A1
公开(公告)日：2014-08-07
申请号：US14233435
申请日：2012-07-27
申请人： Kimberly S. Sampson , Deepa Balasubramanian , Duane A. Lehtinen
发明人： Kimberly S. Sampson , Deepa Balasubramanian , Duane A. Lehtinen
IPC分类号： C12N15/82 , A01H5/10 , A01H5/12 , C07K14/195
CPC分类号： C12N15/8286 , A01H5/10 , A01H5/12 , C07K14/195 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列，SEQ ID NO：1所示核苷酸序列以及变体和片段的核苷酸序列的核酸分子其中。

28. 发明申请

US20140196175A1 AXMI221z, AXMI222z, AXMI223z, AXMI224z and AXMI225z DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译： AXMI221z，AXMI222z，AXMI223z，AXMI224z和AXMI225z DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20140196175A1
公开(公告)日：2014-07-10
申请号：US14207950
申请日：2014-03-13
申请人： Athenix Corp.
发明人： Kimberly Sampson , Daniel Tomso
IPC分类号： C07K16/12 , C12N15/82
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , C07K16/1278 , C12N15/8279 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-32, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-32所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-20所示的核苷酸序列，以及变体和片段。

29. 发明授权

US08686124B2 AXMI223z delta-endotoxin gene and methods for use 有权
标题翻译： AXMI223Zδ-内毒素基因及其使用方法
公开(公告)号：US08686124B2
公开(公告)日：2014-04-01
申请号：US13030415
申请日：2011-02-18
申请人： Kimberly Sampson , Daniel Tomso
发明人： Kimberly Sampson , Daniel Tomso
IPC分类号： A01H5/00 , C12N15/82 , C12N15/00 , C12N5/00 , C12P21/00 , A01N63/00
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , C07K16/1278 , C12N15/8279 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity, including against lepidopteran pests, to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:27 and 28, or the nucleotide sequence set forth in SEQ ID NO:3, 8, 13, and 18, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性（包括防鳞翅目害虫）的组合物和方法。提供了包含毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：27和28所示的氨基酸序列的核苷酸序列或SEQ ID NO：3,8,13和18所示的核苷酸序列，以及其变体和片段。

30. 发明授权

US08450267B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08450267B2
公开(公告)日：2013-05-28
申请号：US12856082
申请日：2010-08-13
申请人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C07K14/00 , C12N15/11
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

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