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    • 69. 发明授权
    • Phospholipid-based inhalation system
    • 基于磷脂的吸入系统
    • US08591954B2
    • 2013-11-26
    • US13532473
    • 2012-06-25
    • Shailaja SomarajuJulie SumanSudipta GangulyRichard Dalby
    • Shailaja SomarajuJulie SumanSudipta GangulyRichard Dalby
    • A61K9/00A61K9/12A61K9/14A61M11/00A61M11/02A61M11/04
    • A61K9/008A61K9/0082A61M15/009A61M2202/0488A61M2209/02
    • The present invention includes a method for delivering medications deeper into the lungs and to the medications' pulmonary targets, which include bronchioles and alveoli. A first particularly preferred embodiment of the invention describes the use of two steps for delivery of a medication. In a first step, an aerosolized therapeutic composition or medication is administered into a patient's respiratory tract, wherein the patient may be any animal or human subject. Following the first step, an aerosolized surfactant is administered into the patient's respiratory tract that facilitates delivery of the aerosolized medication of the first step to the medication's pulmonary target. Another embodiment of the present invention contemplates an apparatus for the delivery of an aerosolized surfactant used that facilitates delivery of previously inhaled aerosolized medication.
    • 本发明包括用于将药物更深地输送到肺部和药物的肺部靶(包括细支气管和肺泡)的方法。 本发明的第一个特别优选的实施方案描述了用于递送药物的两个步骤的用途。 在第一步骤中,将雾化的治疗组合物或药物施用于患者的呼吸道,其中所述患者可以是任何动物或人类受试者。 在第一步之后,将雾化的表面活性剂施用到患者的呼吸道中,其有助于将第一步的雾化药物递送到药物的肺靶。 本发明的另一个实施方案考虑了用于递送雾化表面活性剂的装置,其便于先前吸入的雾化药物的递送。
    • 70. 发明授权
    • Enhanced gene expression in algae
    • 藻类中增强的基因表达
    • US08476019B2
    • 2013-07-02
    • US13504741
    • 2010-11-01
    • George OylerJulian Rosenberg
    • George OylerJulian Rosenberg
    • C12Q1/68
    • C12N15/79C07K14/415C12N15/8216
    • The invention provides a system of enhancing the expression of transgenes in algae. Transgenes are engineered to have a binding site for certain proteins in proximity to their promoter, for example a LexA binding site. The algae is also engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.
    • 本发明提供增强藻类中转基因表达的系统。 转基因被设计为具有接近其启动子的某些蛋白质的结合位点,例如LexA结合位点。 藻类也被设计成表达与蛋白质融合的核小体变异蛋白质,其与DNA结合位点具有亲和性,起协调作用。 一个例子是LexA-p300融合蛋白,其中p300衍生自衣藻。 LexA结合结构域将p300引导到结合位点,p300通过在转基因附近乙酰化组蛋白来松开核小体结构,从而重建局部染色质结构以允许高水平表达。