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71. 发明授权

US08383370B2 Modified RNA ligase for efficient 3′ modification of RNA 有权
公开(公告)号：US08383370B2
公开(公告)日：2013-02-26
申请号：US12525176
申请日：2008-01-30
申请人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina Lin
发明人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina Lin
IPC分类号： C12P19/34 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P19/34 , C12N9/93
摘要： The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase.

72. 发明授权

US08247543B2 Human microRNAs and methods for inhibiting same 有权
标题翻译：人类微小RNA及其抑制方法
公开(公告)号：US08247543B2
公开(公告)日：2012-08-21
申请号：US11919393
申请日：2006-05-01
申请人： Thomas Tuschl , Pablo Landgraf
发明人： Thomas Tuschl , Pablo Landgraf
IPC分类号： C07H21/04 , C07H21/02 , C12N15/63
CPC分类号： C12N15/113 , C12N2310/14 , C12N2330/10
摘要： The invention relates to isolated DNA or RNA molecules comprising at least ten contiguous bases having a sequence in a microRNA shown in SEQ ID NOs: 1-94; 281-374; 467-481; 497-522; or 549, except that up to thirty percent of the bases may be wobble bases, and up to 10% of the contiguous bases may be non-complementary. The invention further relates to modified single stranded microRNA molecules, isolated single stranded anti-microRNA molecules and isolated microRNP molecules. In another embodiment, the invention relates to a method for inhibiting microRNP activity in a cell.
摘要翻译：本发明涉及分离的DNA或RNA分子，其包含至少10个具有SEQ ID NO：1-94所示的微小RNA中的序列的连续碱基; 281-374; 467-481; 497-522; 或549，除了多达30％的碱基可以是摆动碱基，并且多达10％的连续碱基可以是非互补的。本发明还涉及修饰的单链microRNA分子，分离的单链抗微RNA分子和分离的microRNP分子。在另一个实施方案中，本发明涉及抑制细胞中microRNP活性的方法。

73. 发明申请

US20120029061A1 RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE 有权
标题翻译： RNA干扰RNA序列特异性介质
公开(公告)号：US20120029061A1
公开(公告)日：2012-02-02
申请号：US12897740
申请日：2010-10-04
申请人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
发明人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
IPC分类号： A61K31/713 , C07H21/02
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.
摘要翻译：本发明涉及果蝇体外系统，其用于证明dsRNA被加工成长度为21-23个核苷酸（nt）的RNA片段。此外，当这些21-23个片段被纯化并加回到果蝇提取物时，它们在不存在长dsRNA的情况下介导RNA干扰。因此，这些21-23个片段是RNA降解的序列特异性介质。必须在这21-23个片段中存在可能是其特异性长度的分子信号，以招募涉及RNAi的细胞因子。本发明包括这些21-23个片段及其用于特异性失活基因功能的用途。使用这些片段（或具有相同或相似性质的化学合成的寡核苷酸）使得能够靶向用于哺乳动物细胞降解的特异性mRNA，其中使用长dsRNA引发RNAi通常是不实际的，可能是因为有害影响干扰素反应。特定基因功能的特异性靶向在功能基因组和治疗应用中是有用的。

74. 发明申请

US20110281931A1 RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE 有权
公开(公告)号：US20110281931A1
公开(公告)日：2011-11-17
申请号：US12897756
申请日：2010-10-04
申请人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
发明人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
IPC分类号： A61K31/713 , C07H21/02 , C12N5/07
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.

75. 发明申请

US20110244523A1 Modified RNA Ligase for Efficient 3` Modification of RNA 有权
标题翻译：用于高效3'RNA修饰的修饰的RNA连接酶
公开(公告)号：US20110244523A1
公开(公告)日：2011-10-06
申请号：US12525176
申请日：2008-01-30
申请人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina P. Lin
发明人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina P. Lin
IPC分类号： C12P19/34 , C12N9/00
CPC分类号： C12P19/34 , C12N9/93
摘要： The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase.
摘要翻译：本发明提供了一种新的截短突变T4 RNA连接酶2.此外，提供了使用连接酶在不存在ATP的情况下将前腺苷酰供体分子连接到RNA的3'羟基的方法。

76. 发明申请

US20110244446A1 RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE 有权
公开(公告)号：US20110244446A1
公开(公告)日：2011-10-06
申请号：US12897749
申请日：2010-10-04
申请人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
发明人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.

77. 发明申请

US20100233706A1 METHODS TO FIX AND DETECT NUCLEIC ACIDS 有权
标题翻译：固定和检测核酸的方法
公开(公告)号：US20100233706A1
公开(公告)日：2010-09-16
申请号：US12721550
申请日：2010-03-10
申请人： Thomas Tuschl , John Pena , Pavol Cekan
发明人： Thomas Tuschl , John Pena , Pavol Cekan
IPC分类号： C12Q1/68 , C12N5/00
CPC分类号： G01N1/30 , C12Q1/6806 , C12Q1/6841 , C12Q2525/207 , C12Q2523/101
摘要： In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.
摘要翻译：一方面，本发明涉及用于将短核酸固定在生物样品中的方法。另一方面，本发明涉及用于检测生物样品中靶目标短核酸的方法。该方法包括使生物样品与含醛固定剂接触，随后使样品与水溶性碳二亚胺接触。另一方面，本发明涉及用于将短核酸固定在生物样品中的试剂盒。该套件包括用于保持样品的支撑衬底; 含醛固定剂; 和水溶性碳二亚胺。

78. 发明授权

US07772389B2 Anti-microRNA oligonucleotide molecules 有权
标题翻译：抗microRNA寡核苷酸分子
公开(公告)号：US07772389B2
公开(公告)日：2010-08-10
申请号：US10589449
申请日：2005-02-11
申请人： Thomas Tuschl , Markus Landthaler , Gunter Meister , Sebastien Pfeffer
发明人： Thomas Tuschl , Markus Landthaler , Gunter Meister , Sebastien Pfeffer
IPC分类号： C07H21/04
CPC分类号： C12N15/113 , C12N2310/113 , C12N2310/141 , C12N2310/3145 , C12N2310/315 , C12N2310/321 , C12N2310/3521 , C12N2310/533
摘要： The invention relates to isolated anti-microRNA molecules. In another embodiment, the invention relates to an isolated microRNA molecule. In yet another embodiment, the invention provides a method for inhibiting microRNP activity in a cell.
摘要翻译：本发明涉及分离的抗微小RNA分子。在另一个实施方案中，本发明涉及分离的微小RNA分子。在另一个实施方案中，本发明提供了抑制细胞中的microRNP活性的方法。

79. 发明申请

US20060166910A1 Rna-interference by single-stranded rna molecules 失效
标题翻译：单链rna分子的Rna-干扰
公开(公告)号：US20060166910A1
公开(公告)日：2006-07-27
申请号：US10520470
申请日：2003-07-10
申请人： Thomas Tuschl , Javier Martinez , Henning Urlaub , Reinhard Luhrmann
发明人： Thomas Tuschl , Javier Martinez , Henning Urlaub , Reinhard Luhrmann
IPC分类号： A61K48/00
CPC分类号： C12N15/113 , A61K47/549 , A61K47/557 , C07K14/4705 , C07K19/00 , C12N15/111 , C12N2310/14 , C12N2310/351 , C12N2310/3513 , C12N2320/30 , C12N2320/51 , C12N2330/30
摘要： The present invention relates to sequence and structural features of single-stranded (ss) RNA molecules required to mediate target-specific nucleic acid modifications by RNA-interference (RNAi), such as target mRNA degradation and/or DNA methylation.
摘要翻译：本发明涉及通过RNA干扰（RNAi）介导靶特异性核酸修饰所需的单链（ss）RNA分子的序列和结构特征，例如靶mRNA降解和/或DNA甲基化。

80. 发明授权

US08957197B2 RNA sequence-specific mediators of RNA interference 有权
公开(公告)号：US08957197B2
公开(公告)日：2015-02-17
申请号：US13008636
申请日：2011-01-18
申请人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
发明人： Thomas Tuschl , Phillip D. Zamore , Phillip A. Sharp , David P. Bartel
IPC分类号： C07H21/04 , C07H21/02
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.

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