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71. 发明授权

US08309332B2 GRG23 and GRG51 polypeptides having herbicide resistance activity 有权
标题翻译：具有除草剂抗性活性的GRG23和GRG51多肽
公开(公告)号：US08309332B2
公开(公告)日：2012-11-13
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

72. 发明授权

US08252980B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252980B2
公开(公告)日：2012-08-28
申请号：US12856021
申请日：2010-08-13
申请人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 31, or the nucleotide sequence set forth in SEQ ID NO: 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：31所示的氨基酸序列的核苷酸序列或SEQ ID NO：30所示的核苷酸序列的分离的多核苷酸。

73. 发明授权

US08173590B2 AXMI-004, a delta-endotoxin gene and methods for its use 有权
公开(公告)号：US08173590B2
公开(公告)日：2012-05-08
申请号：US12032479
申请日：2008-02-15
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01N37/18 , C07K14/375
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.

74. 发明授权

US08147856B2 AXMI-031, AXMI-039, AXMI-040 and AXMI-049, a family of novel delta-endotoxin genes and methods for their use 有权
标题翻译： AXMI-031，AXMI-039，AXMI-040和AXMI-049，一种新型的δ-内毒素基因及其使用方法
公开(公告)号：US08147856B2
公开(公告)日：2012-04-03
申请号：US12364335
申请日：2009-02-02
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
IPC分类号： A01N63/02 , C12N15/32 , C12P21/04 , C07K14/325
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

75. 发明授权

US08101825B2 AXMI-004, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-004，一种δ-内毒素基因及其使用方法
公开(公告)号：US08101825B2
公开(公告)日：2012-01-24
申请号：US12032445
申请日：2008-02-15
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：3和5所示的氨基酸序列的核苷酸序列和SEQ ID NO：1,2和4所示的核苷酸序列的分离的核酸分子，以及作为其变体和片段。

76. 发明授权

US08097775B2 GRO-1 herbicide resistance gene and methods for its use 有权
标题翻译： GRO-1除草剂抗性基因及其使用方法
公开(公告)号：US08097775B2
公开(公告)日：2012-01-17
申请号：US12352532
申请日：2009-01-12
申请人： Philip E. Hammer , Todd K. Hinson , Amy Elizabeth Shekita
发明人： Philip E. Hammer , Todd K. Hinson , Amy Elizabeth Shekita
IPC分类号： C12N15/52 , C12N15/82
CPC分类号： C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequence set forth in SEQ ID NO:1, 2, or 4.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,2或4所示的核苷酸序列的分离的核酸分子。

77. 发明授权

US08003854B2 GRG32: a novel EPSP synthase gene conferring herbicide resistance 有权
标题翻译： GRG32：赋予除草剂抗性的新型EPSP合成酶基因
公开(公告)号：US08003854B2
公开(公告)日：2011-08-23
申请号：US11769255
申请日：2007-06-27
申请人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
发明人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，本发明提供了分离的核酸分子，其包含SEQ ID NO：1或14所示的核苷酸序列，编码SEQ ID NO：2所示氨基酸序列的核苷酸序列，作为登录号NRRL B-30931的细菌宿主，以及其变体和片段。

78. 发明授权

US07989679B2 Methods and compositions for improved enzyme activity in transgenic plants 失效
标题翻译：在转基因植物中改善酶活性的方法和组合物
公开(公告)号：US07989679B2
公开(公告)日：2011-08-02
申请号：US11681285
申请日：2007-03-02
申请人： Michael G. Koziel , Laura Cooper Schouten , Brian Vande Berg
发明人： Michael G. Koziel , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于增加植物，植物细胞，组织和种子中广泛生理光谱的酶活性的组合物和方法。组合物包括植物或植物部分，其包含两个或更多个编码与更多生理光谱相关的多肽的多核苷酸，而不是单个表达。还提供了包含这些多核苷酸分子的载体以及包含载体的宿主细胞。组合物还包括转化的细菌，植物，植物细胞，组织和种子。此外，提供了用于生产本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物中除草剂抗性的方法。

79. 发明授权

US07919272B2 Methods and compositions for controlling plant pests 有权
标题翻译：防治植物害虫的方法和组成
公开(公告)号：US07919272B2
公开(公告)日：2011-04-05
申请号：US12718059
申请日：2010-03-05
申请人： Kimberly S. Sampson , Daniel John Tomso
发明人： Kimberly S. Sampson , Daniel John Tomso
IPC分类号： C12P21/06 , C07K14/00
CPC分类号： C07K14/325 , A01N37/46 , C07K14/32 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24, or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：13-24所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12和25-44所示的核苷酸序列，作为以及其变体和片段。

80. 发明授权

US07910805B2 Bacterial glutamine synthetases and methods of use 有权
标题翻译：细菌性谷氨酰胺合成酶及其使用方法
公开(公告)号：US07910805B2
公开(公告)日：2011-03-22
申请号：US11760602
申请日：2007-06-08
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： C12N15/52 , C12N15/82
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

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