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1. 发明申请

US20170166954A1 METHODS FOR DETECTING NUCLEIC ACID FRAGMENTS 审中-公开
公开(公告)号：US20170166954A1
公开(公告)日：2017-06-15
申请号：US15025223
申请日：2014-09-25
申请人： BIO-ID DIAGNOSTIC INC.
发明人： Thuraiayah VINAYAGAMOORTHY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q1/6858 , C12Q1/6886 , C12Q2600/156 , C12Q2525/186 , C12Q2525/155 , C12Q2525/161 , C12Q2525/207 , C12Q2525/307 , C12Q2531/113 , C12Q2535/122 , C12Q2535/125
摘要： Provided are methods for detecting and analyzing polynucleotides in a biological sample or sample derived therefrom for example, using a synthetic template polynucleotide. In some aspects, a target polynucleotide in the sample hybridizes to the template polynucleotide and is extended by a polymerase, generating an extended target polynucleotide. In some examples, the extended target polynucleotide is amplified, for example, by polymerase chain reaction, and sequences of the target polynucleotide determined, for example, by priming in the region of the extended target polynucleotide generated by extension and sequencing towards the region having identity to the target polynucleotide. In some aspects, the target polynucleotide is thereby detected in the sample and its sequence identified. In some aspects, the provided methods can be used to capture polynucleotide fragments in a biological sample, for example, plasma, and determine respective biomarkers they carry, for example, for cancer diagnosis and prognosis.

2. 发明授权

US07727745B2 Synthesis of single-stranded DNA 有权
标题翻译：单链DNA的合成
公开(公告)号：US07727745B2
公开(公告)日：2010-06-01
申请号：US11464273
申请日：2006-08-14
申请人： Thuraiayah Vinayagamoorthy
发明人： Thuraiayah Vinayagamoorthy
IPC分类号： C12P19/34 , C07H21/02
CPC分类号： C12P19/34 , C12N15/10
摘要： A method of producing single-stranded DNA. In one form, the method involves providing a uracil-containing oligonucleotide template molecule having a sequence that is complementary to a part of a target single-stranded DNA molecule of length greater than the template molecule; providing one or more parts of the target molecule including a base sequence complementary to a part of the template molecule; annealing the part(s) of the target molecule to the template molecule and forming the complete target molecule by ligating together at least two adjacent parts of the target molecule while annealed to the template molecule, and/or extending at least one part of the target molecule to form a sequence complementary to a remainder of the template molecule by nucleotide polymerization, and then separating the template molecule from the target molecule. In another form, an intermediate molecule is annealed to the template and then enzymatically cut, and one part is then extended by DNA polymerization using monomers of increased molecular weight or ionic charge compared to the monomers used to form the intermediate molecule.
摘要翻译：一种单链DNA的制备方法。在一种形式中，该方法包括提供含有尿嘧啶的寡核苷酸模板分子，其具有与长度大于模板分子的目标单链DNA分子部分互补的序列; 提供靶分子的一个或多个部分，包括与模板分子的一部分互补的碱基序列; 将靶分子的部分退火至模板分子，并通过将靶分子的至少两个相邻部分连接在一起，同时与模板分子退火而形成完整的靶分子，和/或延伸靶的至少一部分分子通过核苷酸聚合形成与模板分子的其余部分互补的序列，然后将模板分子与靶分子分离。在另一种形式中，将中间体分子与模板退火，然后进行酶切，然后通过与用于形成中间分子的单体相比增加分子量或离子电荷的单体通过DNA聚合延伸一部分。

3. 发明授权

US08785130B2 Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material 有权
标题翻译：使用包括基于核苷酸序列的代码的标记来监测遗传物质的检测和鉴定方法
公开(公告)号：US08785130B2
公开(公告)日：2014-07-22
申请号：US11481046
申请日：2006-07-06
申请人： Thuraiayah Vinayagamoorthy
发明人： Thuraiayah Vinayagamoorthy
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6869 , C12Q1/6848 , C12Q1/686
摘要： Disclosed is the use of artificially-generated nucleic acid coded markers to monitor nucleic acid amplification and sequencing reactions designed to detect or analyze biological samples. The markers generally include, along with a unique sequence preferably including coded section designed to represent one or more factors of interest, primer annealing sequences so that the marker may be amplified and sequenced in the same process and using the same amplification and sequencing primers as for the sample target. The invention also relates to the marker itself, and other uses, such as identifying the origin of various materials or products.
摘要翻译：公开了人造产生的核酸编码标记物用于监测设计用于检测或分析生物样品的核酸扩增和测序反应的用途。标记通常包括优选包括被设计成代表一个或多个感兴趣因子的编码区的唯一序列，引物退火序列，使得标记可以在相同方法中扩增和测序，并使用相同的扩增和测序引物样本目标。本发明还涉及标记本身以及其他用途，例如识别各种材料或产品的来源。

4. 发明申请

US20080003573A1 Determination of variants produced upon replication or transcription of nucleic acid sequences 有权
标题翻译：确定在核酸序列的复制或转录时产生的变体
公开(公告)号：US20080003573A1
公开(公告)日：2008-01-03
申请号：US11475921
申请日：2006-06-28
申请人： Thuraiayah Vinayagamoorthy
发明人： Thuraiayah Vinayagamoorthy
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6813
摘要： A method of determining whether or not a nucleic acid having an expected sequence or one or more variants of the expected sequence are present in a sample containing nucleic acids after replication, transcription or editing (or other transformation) of a substrate nucleic acid. The method involves deciding an expected sequence likely to be formed in the sample upon the replication, transcription or editing of the substrate nucleic acid, and possible variants of the expected sequence, providing primer pairs for a polymerase chain reaction, reverse transcriptase polymerase chain reaction or ligase chain reaction, carrying out the polymerase chain reaction or reverse transcriptase polymerase chain reaction in one or more steps to form amplicons, and analyzing the amplicons to determining whether or not a nucleic acid having the expected sequence and/or variants are present in the sample. The primers of the primer pairs are designed to anneal to regions of the nucleic acid of the expected sequence and the variants, the regions being selected to reveal unambiguously the presence or absence in the sample of the nucleic acid of the expected sequence or the variants thereof according to the presence or absence of specific amplicons amplified by the primers.
摘要翻译：一种确定具有预期序列或预期序列的一个或多个变体的核酸是否存在于含有底物核酸的复制，转录或编辑（或其他转化）之后的核酸的样品中的方法。该方法涉及确定在底物核酸的复制，转录或编辑时可能在样品中形成的预期序列，以及预期序列的可能变体，提供聚合酶链式反应的引物对，逆转录酶聚合酶链式反应或连接酶链反应，在一个或多个步骤中进行聚合酶链反应或逆转录酶聚合酶链反应以形成扩增子，并分析扩增子以确定样品中是否存在具有预期序列和/或变体的核酸。引物对的引物被设计为退火到期望序列和变体的核酸区域，该区域被选择以明确地揭示预期序列或其变体的核酸样品中的存在或不存在根据引物扩增的特异扩增子的存在或不存在。

5. 发明授权

US11028442B2 Simultaneous detection of multiple nucleic acid templates using modified primers 有权
公开(公告)号：US11028442B2
公开(公告)日：2021-06-08
申请号：US16521965
申请日：2019-07-25
申请人： Bio-ID Diagnostic Inc.
发明人： Thuraiayah Vinayagamoorthy
IPC分类号： C12Q1/68 , C12Q1/6883
摘要： The invention refers to a method of detecting one or more deoxyribose nucleotide (e.g. DNA) template(s) in a heterogenous sample by generation of template specific surrogate nucleotide sequences. This invention is applied to but not restricted in detecting genetic variations including cancer markers and pathogens.

6. 发明授权

US09150906B2 Determination of variants produced upon replication or transcription of nucleic acid sequences 有权
标题翻译：确定在核酸序列的复制或转录时产生的变体
公开(公告)号：US09150906B2
公开(公告)日：2015-10-06
申请号：US11475921
申请日：2006-06-28
申请人： Thuraiayah Vinayagamoorthy
发明人： Thuraiayah Vinayagamoorthy
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6813
摘要： A method of determining whether or not a nucleic acid having an expected sequence or one or more variants of the expected sequence are present in a sample containing nucleic acids after replication, transcription or editing (or other transformation) of a substrate nucleic acid. The method involves deciding an expected sequence likely to be formed in the sample upon the replication, transcription or editing of the substrate nucleic acid, and possible variants of the expected sequence, providing primer pairs for a polymerase chain reaction, reverse transcriptase polymerase chain reaction or ligase chain reaction, carrying out the polymerase chain reaction or reverse transcriptase polymerase chain reaction in one or more steps to form amplicons, and analyzing the amplicons to determining whether or not a nucleic acid having the expected sequence and/or variants are present in the sample. The primers of the primer pairs are designed to anneal to regions of the nucleic acid of the expected sequence and the variants, the regions being selected to reveal unambiguously the presence or absence in the sample of the nucleic acid of the expected sequence or the variants thereof according to the presence or absence of specific amplicons amplified by the primers.
摘要翻译：一种确定具有预期序列或预期序列的一个或多个变体的核酸是否存在于含有底物核酸的复制，转录或编辑（或其他转化）之后的核酸的样品中的方法。该方法涉及确定在底物核酸的复制，转录或编辑时可能在样品中形成的预期序列，以及预期序列的可能变体，提供聚合酶链式反应的引物对，逆转录酶聚合酶链式反应或连接酶链反应，在一个或多个步骤中进行聚合酶链反应或逆转录酶聚合酶链反应以形成扩增子，并分析扩增子以确定样品中是否存在具有预期序列和/或变体的核酸。引物对的引物被设计为退火到期望序列和变体的核酸区域，该区域被选择以明确地揭示预期序列或其变体的核酸样品中的存在或不存在根据引物扩增的特异扩增子的存在或不存在。

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