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1. 发明申请

US20220162673A1 METHODS FOR PREPARATIVE IN VITRO CLONING 有权
公开(公告)号：US20220162673A1
公开(公告)日：2022-05-26
申请号：US17536828
申请日：2021-11-29
申请人： Gen9, Inc.
发明人： Li-Yun A. Kung , Daniel Schindler , Joseph Jacobson
IPC分类号： C12Q1/6806 , C12N15/10 , C12Q1/6855
摘要： Methods and devices relate to the isolation of nucleic acids of interest from within a population of nucleic acids such as libraries of nucleic acid sequences.

2. 发明授权

US11242523B2 Compositions, methods and apparatus for oligonucleotides synthesis 有权
公开(公告)号：US11242523B2
公开(公告)日：2022-02-08
申请号：US16373587
申请日：2019-04-02
申请人： Gen9, Inc.
发明人： Giles F. Hall , Scott S. Lawton
IPC分类号： C12N15/10 , C12P19/34 , C12N15/66 , C40B40/06
摘要： Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.

3. 发明申请

US20210254052A1 METHODS AND DEVICES FOR HIGH FIDELITY POLYNUCLEOTIDE SYNTHESIS 有权
公开(公告)号：US20210254052A1
公开(公告)日：2021-08-19
申请号：US17242178
申请日：2021-04-27
申请人： Gen9, Inc.
发明人： Joseph Jacobson , George M. Church , Larry Li-Yang Chu
IPC分类号： C12N15/10 , B01L3/02 , B01L3/00 , C12Q1/6837 , C12Q1/6844 , C12N15/66
摘要： Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

4. 发明授权

US11084014B2 Methods and devices for nucleic acids synthesis 有权
公开(公告)号：US11084014B2
公开(公告)日：2021-08-10
申请号：US16533892
申请日：2019-08-07
申请人： Gen9, Inc.
发明人： Joseph Jacobson , Li-yun A. Kung , Andrew Kirk Wilson , Senthil Ramu , Daniel Schindler , Michael Hudson
IPC分类号： C12Q1/68 , B01J19/00 , C12N15/10 , C12N15/66 , C12P19/34
摘要： Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.

5. 发明申请

US20200181603A1 MICROARRAY SYNTHESIS AND ASSEMBLY OF GENE-LENGTH POLYNUCLEOTIDES 审中-公开
公开(公告)号：US20200181603A1
公开(公告)日：2020-06-11
申请号：US16793632
申请日：2020-02-18
申请人： Gen9, Inc.
发明人： Andrew V. Oleinikov
IPC分类号： C12N15/10 , B82Y30/00 , C40B80/00 , B01J19/00 , C40B50/14 , C12Q1/6837
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

6. 发明申请

US20200181591A1 COMPOSITIONS AND METHODS FOR SITE-DIRECTED DNA NICKING AND CLEAVING 审中-公开
公开(公告)号：US20200181591A1
公开(公告)日：2020-06-11
申请号：US16713529
申请日：2019-12-13
申请人： Gen9, Inc.
发明人： Joseph Jacobson , Ishtiaq Saaem , Michael E. Hudson , Devin Leake
IPC分类号： C12N9/22 , C12N15/62 , C12N15/66 , C12N15/10 , C12P19/34
摘要： Aspects of the disclosure related to compositions and methods for site-directed DNA nicking and/or cleaving, and use thereof in, for example, polynucleotide assembly.

7. 发明授权

US10202608B2 Iterative nucleic acid assembly using activation of vector-encoded traits 有权
公开(公告)号：US10202608B2
公开(公告)日：2019-02-12
申请号：US13250083
申请日：2011-09-30
申请人： William J. Blake
发明人： William J. Blake
IPC分类号： C12Q1/68 , C12N15/66 , C12Q1/6844 , C12N15/10 , C12N15/64
摘要： Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.

8. 发明授权

US09023601B2 Microarray synthesis and assembly of gene-length polynucleotides 有权
公开(公告)号：US09023601B2
公开(公告)日：2015-05-05
申请号：US13250207
申请日：2011-09-30
申请人： Andrew V. Oleinikov
发明人： Andrew V. Oleinikov
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C40B50/14 , B01J19/00 , B82Y30/00 , C40B80/00 , C40B40/06
CPC分类号： C12N15/1068 , B01J19/0046 , B01J2219/00378 , B01J2219/00385 , B01J2219/00432 , B01J2219/00454 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00626 , B01J2219/00639 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B80/00
摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

9. 发明授权

US08716467B2 Methods and devices for nucleic acid synthesis 有权
标题翻译：用于核酸合成的方法和装置
公开(公告)号：US08716467B2
公开(公告)日：2014-05-06
申请号：US13038714
申请日：2011-03-02
申请人： Joseph Jacobson
发明人： Joseph Jacobson
IPC分类号： C07H21/00
CPC分类号： C12P19/34 , C12M1/00 , C12N15/1068
摘要： Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.
摘要翻译：公开了用于合成寡核苷酸文库的多核苷酸和多核苷酸文库的装置和方法。在示例性实施例中，该装置包括具有多个特征的支撑件。每个特征包含多个寡核苷酸。在每个特征中，多个寡核苷酸中的每一个包括X核苷的相同的预定亚基序列和Y核苷的简并序列。可以使用特征子集的预定组合来产生具有预定的Z核苷序列的多核苷酸。

10. 发明申请

US20130309725A1 Methods and Devices for In Situ Nucleic Acid Synthesis 有权
标题翻译：用于原位核酸合成的方法和装置
公开(公告)号：US20130309725A1
公开(公告)日：2013-11-21
申请号：US13700291
申请日：2011-05-26
申请人： Joseph Jacobson , Senthil Ramu , Daniel Schindler
发明人： Joseph Jacobson , Senthil Ramu , Daniel Schindler
IPC分类号： C12P19/34
CPC分类号： C12N15/1068 , C12N15/1093 , C12N15/66 , C12P19/34
摘要： Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.
摘要翻译：公开了用于原位合成核酸的组合物，方法和装置。在一个示例性实施方案中，通过部分双链寡核苷酸的杂交，连接和去除不需要的核苷酸，加入一个或多个核苷酸来延伸载体结合的寡核苷酸。

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