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1. 发明授权

US09279130B2 Methods for manufacturing plant cell walls comprising chitin 有权
标题翻译：制造包含壳多糖的植物细胞壁的方法
公开(公告)号：US09279130B2
公开(公告)日：2016-03-08
申请号：US13522506
申请日：2011-01-24
申请人： Marc De Block , Frank Meulewaeter , Vincent Bulone , Gea Guerriero
发明人： Marc De Block , Frank Meulewaeter , Vincent Bulone , Gea Guerriero
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/05 , C12N9/10
CPC分类号： C12N15/8246 , C12N9/1051 , C12Y204/01016
摘要： Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants.
摘要翻译：提供了用于修饰植物二次细胞壁的反应性的方法和手段，特别是在棉纤维中发现的棉花细胞壁中。这可以通过在棉花植物中表达编码Saprolegnia monoica几丁质合酶的嵌合基因来方便地实现。

2. 发明授权

US09228194B2 Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes 有权
公开(公告)号：US09228194B2
公开(公告)日：2016-01-05
申请号：US13361159
申请日：2012-01-30
申请人： Ralph E. Dewey , Balazs Siminszky , Steven W. Bowen , Lily Gavilano
发明人： Ralph E. Dewey , Balazs Siminszky , Steven W. Bowen , Lily Gavilano
IPC分类号： A01H1/06 , A01H5/12 , C12N15/05 , C12N9/06 , C12N15/82 , C12N9/02
CPC分类号： A01H5/12 , A01H1/06 , C12N9/0073 , C12N15/8243 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

3. 发明授权

US08951791B2 System for expression of genes in plants 有权
公开(公告)号：US08951791B2
公开(公告)日：2015-02-10
申请号：US14093833
申请日：2013-12-02
申请人： iBio, Inc.
发明人： Oleg Fedorkin , Shailaja Rabindran , Vidadi Yusibov
IPC分类号： C12N15/05 , C12N15/82 , C07K14/61 , C07K14/62
CPC分类号： C12N15/8203 , C07K14/61 , C07K14/62 , C12N7/00 , C12N15/8257 , C12N2770/00043 , C12P21/02
摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

4. 发明授权

US08772577B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08772577B2
公开(公告)日：2014-07-08
申请号：US12943085
申请日：2010-11-10
申请人： Andre R Abad , Hua Dong , Sue B Lo , Xiaomei Shi , Thomas Chad Wolfe
发明人： Andre R Abad , Hua Dong , Sue B Lo , Xiaomei Shi , Thomas Chad Wolfe
IPC分类号： A01H5/00 , C12N15/82 , C12N15/05 , C12N15/32 , C12P21/00 , C07K14/325
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

5. 发明授权

US08722988B2 Soybean cultivar S100242 有权
标题翻译：大豆品种S100242
公开(公告)号：US08722988B2
公开(公告)日：2014-05-13
申请号：US13559026
申请日：2012-07-26
申请人： Justin T. Mason
发明人： Justin T. Mason
IPC分类号： A01H5/00 , A01H5/10 , A01H4/00 , A01H1/00 , A01H1/06 , C12N15/82 , C12N5/04 , C12N15/05
CPC分类号： A01H5/10 , C11B1/00
摘要： A soybean cultivar designated S100242 is disclosed. The invention relates to the seeds of soybean cultivar S100242, to the plants of soybean cultivar S100242, to the plant parts of soybean cultivar S100242, and to methods for producing progeny of soybean cultivar S100242. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S100242. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S100242, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S100242 with another soybean cultivar.
摘要翻译：公开了一种称为S100242的大豆品种。本发明涉及大豆品种S100242的种子，大豆品种S100242的植物，大豆品种S100242的植物部位，以及大豆品种S100242的后代的生产方法。本发明还涉及含有一种或多种转基因的遗传物质的大豆植物的制备方法以及通过这些方法制备的转基因大豆植物和植物部分的方法。本发明还涉及大豆品种或繁殖品种，以及来源于大豆品种S100242的植物成分。本发明还涉及从大豆品种S100242得到的其他大豆品种，品系，或植物成分的制造方法，以及从使用这些方法得到的大豆植物，品种及其成分的方法。本发明还涉及杂交大豆种子，植物和通过品种S100242与另一大豆品种杂交产生的植物部分。

6. 发明授权

US08697949B2 Temporal regulation of gene expression by MicroRNAs 有权
标题翻译： MicroRNAs基因表达的时间调控
公开(公告)号：US08697949B2
公开(公告)日：2014-04-15
申请号：US12665338
申请日：2008-06-26
申请人： Edwards Allen , Sara E. Heisel , Sergey Ivashuta , Elysia Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
发明人： Edwards Allen , Sara E. Heisel , Sergey Ivashuta , Elysia Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
IPC分类号： C12N15/64 , C12N15/00 , C12N15/05 , C12N15/82 , A01H5/00 , C07H21/04
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
摘要： This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
摘要翻译：本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。

7. 发明授权

US08071841B2 Or gene and its use in manipulating carotenoid content and composition in plants and other organisms 有权
标题翻译：或基因及其在操作植物和其他生物体中类胡萝卜素含量和组成的用途
公开(公告)号：US08071841B2
公开(公告)日：2011-12-06
申请号：US11639064
申请日：2006-12-14
申请人： Li Li , Joyce Van Eck
发明人： Li Li , Joyce Van Eck
IPC分类号： C12N15/05 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8243 , C07K14/415 , C12N15/825
摘要： The cauliflower (Brassica oleracea L. var. botrytis) Or gene is a semi-dominant, single-locus mutation. It induces the accumulation of high levels of beta-carotene in various tissues that are normally devoid of carotenoids, turning them orange. Using a map-based cloning strategy, we identified a single gene representing Or and successfully verified its identity by functional complementation in the wild type cauliflower. The Or gene encodes a plastid membrane protein containing the DnaJ zinc figure domain. A likely gain-of-function mutation from a 4.3-kb retrotransposon insertion in the Or allele confers the orange phenotype in the mutant. Southern blot analysis revealed that Or is a single-copy sequence in the cauliflower genome. High level of expression of the Or gene and the protein was found in very young leaves, curds, and flowers at comparable abundance between wild type and the Or mutant. Or likely functions in regulating the differentiation of some non-photosynthetic plastids into chromoplasts, which provide the deposition “sink” for carotenoid accumulation. Successful demonstration of Or in conferring carotenoid accumulation in potato tubers indicates its potential use to improve the nutritional value in staple crops.
摘要翻译：花椰菜（Brassica oleracea L. var。botrytis）或基因是半显性的单基因座突变。它引起高水平的β-胡萝卜素在各种组织中的积累，通常不含类胡萝卜素，使其变成橙色。使用基于地图的克隆策略，我们确定了代表Or的单个基因，并通过在野生花椰菜中的功能互补成功地验证了其身份。 Or基因编码含有DnaJ锌图形结构域的质体膜蛋白。来自Or等位基因中的4.3-kb反转录转座子插入的可能的功能增益赋予突变体中的橙色表型。 Southern印迹分析显示Or是花椰菜基因组中的单拷贝序列。在野生型和Or突变体之间的相当丰度的非常年轻的叶子，凝乳和花中发现了Or基因和蛋白质的高水平表达。或者可能发挥调节某些非光合质体分化为色素的作用，为色素沉积提供了沉淀。在马铃薯块茎中成功示范或提供类胡萝卜素积累表明其可能用于改善主要作物的营养价值。

8. 发明授权

US08008548B2 Plants with reduced susceptibility to pathogenic oomycetes 有权
标题翻译：对致病性卵菌的易感性降低的植物
公开(公告)号：US08008548B2
公开(公告)日：2011-08-30
申请号：US11628885
申请日：2005-06-10
申请人： Jacobus Petrus Cornelis De Wit , Cornelis Maria Petrus Van Dun , Johannes Wilhelmus Schut , Petrus Lambertus J. Egelmeers , Robert Helene Ghislain Dirks
发明人： Jacobus Petrus Cornelis De Wit , Cornelis Maria Petrus Van Dun , Johannes Wilhelmus Schut , Petrus Lambertus J. Egelmeers , Robert Helene Ghislain Dirks
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , A01H1/06 , C12N15/01 , C12N15/05
CPC分类号： A01H5/12 , A01H5/02 , A01H6/14
摘要： The present invention relates to a for obtaining a plant showing a reduced susceptibility towards infection with a pathogen, in particular an oomycete, comprising treating MO seeds of a plant species to be modified with a mutagenic agent to obtain M1 seeds and growing plants therefrom the thus obtained M1 seeds to obtain M1 plants, inoculation of the thus obtained M1+n plants with the pathogen and selecting plants that show a reduction or absence of sporulation of the pathogen as plants having a reduced susceptibility phenotype. The invention further relates to plants, seeds, pollen, cells and tissues that have the reduced susceptibility towards oomycetes.
摘要翻译：本发明涉及一种用于获得对病原体特别是卵菌感染易感性降低的植物，其包括用致突变剂处理待修饰的植物物种的MO种子，从而获得M1种子并从中生长植物获得M1种子以获得M1植物，用病原体接种由此获得的M1 + n植物，并选择显示具有减少或不存在病原体孢子形成的植物，作为具有降低的易感性表型的植物。本发明还涉及具有对卵菌的敏感性降低的植物，种子，花粉，细胞和组织。

9. 发明授权

US08008543B2 Modification of flavonoid biosynthesis in plants by PAP1 有权
标题翻译： PAP1修饰植物中类黄酮生物合成
公开(公告)号：US08008543B2
公开(公告)日：2011-08-30
申请号：US12091546
申请日：2006-10-25
申请人： Aidyn Mouradov , German Spangenberg
发明人： Aidyn Mouradov , German Spangenberg
IPC分类号： A01H1/00 , A01H5/00 , C12N5/14 , C12N15/05 , C07H21/04 , C07K14/415
CPC分类号： C12N15/825 , C07K14/415 , C12N15/8243
摘要： The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, more particularly flavonoid biosynthesis-regulating transcription factors, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the present invention relates to a PURPLE ANTHOCYANIN PIGMENT 1 (PAP1) or PAP1-like transcription factor.
摘要翻译：本发明涉及编码类黄酮生物合成酶的核酸，更特别地涉及类黄酮生物合成调节转录因子的核酸及其在植物中修饰黄酮生物合成的用途。本发明还涉及包括这种核酸的构建体和载体以及相关多肽。更具体地说，本发明涉及一种紫草素（PURPLE ANTHOCYANIN）1（PAP1）或PAP1样转录因子。

10. 发明授权

US07557267B2 Rubisco activase with increased thermostability and methods of use thereof 有权
标题翻译：具有增加的热稳定性的Rubisco活化酶及其使用方法
公开(公告)号：US07557267B2
公开(公告)日：2009-07-07
申请号：US11867723
申请日：2007-10-05
申请人： Itzhak Kurek , Lu Liu , Genhai Zhu
发明人： Itzhak Kurek , Lu Liu , Genhai Zhu
IPC分类号： C12N15/05 , A01H1/00 , C07H21/04 , C07K14/415 , C12N15/29
CPC分类号： C12N9/88 , C12N15/8261 , Y02A40/146
摘要： The present invention provides thermostable polypeptides related to Arabidopsis Rubisco Activase polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nuclei acids of the invention to enhance resistance of plants to heat stress are encompassed.
摘要翻译：本发明提供与拟南芥Rubisco活化酶多肽相关的热稳定多肽。还提供了编码本发明多肽的核酸。包括使用本发明的多肽和核酸以增强植物对热应激的抗性的方法。

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