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1. 发明授权

US10584325B2 Cellobiohydrolase variants and polynucleotides encoding same 有权
公开(公告)号：US10584325B2
公开(公告)日：2020-03-10
申请号：US16178286
申请日：2018-11-01
申请人： Novozymes A/S
发明人： Kim Borch , Kenneth Jensen , Kristian Krogh , Brett McBrayer , Peter Westh , Jeppe Kari , Johan Olsen , Trine Sorensen , Michael Windhal , Hui Xu
IPC分类号： C12N9/42 , C12N15/56 , C12N5/10 , C12N15/82 , C12P19/02 , C12P19/14
摘要： The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

2. 发明授权

US10584323B2 Glucoamylase variants 有权
公开(公告)号：US10584323B2
公开(公告)日：2020-03-10
申请号：US15037040
申请日：2014-12-03
申请人： Danisco US Inc.
发明人： Daniel Esteban Torres Pazmino , Viktor Yuryevich Alekseyev , Casper Willem Vroemen , David A. Estell
IPC分类号： C12N9/26 , C12N9/30 , C12P19/14 , C12N1/21 , C12N15/56 , C12N9/34
摘要： Aspects of the present disclosure include glucoamylase (GA) variants having at least one improved property over a parent GA, compositions containing the GA variants, nucleic acids encoding the GA variants, and methods for producing and using the same. In some aspects, the GA variant is a variant of a parent GA from Humicola grisea.

3. 发明授权

US10550376B2 Xylanase 有权
公开(公告)号：US10550376B2
公开(公告)日：2020-02-04
申请号：US15738211
申请日：2016-06-16
申请人： KAO CORPORATION
发明人： Nozomu Shibata , Mari Suetsugu
IPC分类号： C12N15/56 , C12N9/24 , C12P19/02 , C12P19/14 , C12N15/70 , C12N15/75 , C12N15/80
摘要： Provided is a novel xylanase having high xylanase activity. A protein consisting of an amino acid sequence described in the following (a), (b) or (c) and having xylanase activity: (a) the amino acid sequence set forth in positions 23 to 404 of SEQ ID NO: 2; (b) an amino acid sequence having at least 90% identity to the amino acid sequence set forth in positions 23 to 404 of SEQ ID NO: 2; (c) an amino acid sequence modified from the amino acid sequence set forth in positions 23 to 404 of SEQ ID NO: 2 by deletion, insertion, substitution, or addition of one or more amino acids.

4. 发明授权

US10006016B2 Polypeptides having xylanase activity and polynucleotides thereof 有权
公开(公告)号：US10006016B2
公开(公告)日：2018-06-26
申请号：US15495691
申请日：2017-04-24
申请人： Novozymes, Inc.
发明人： Debbie Yaver , Paul Harris , Suzanne Otani , Janine Lin , Hanshu Ding
IPC分类号： C12N15/56 , C12N9/24
CPC分类号： C12N9/2482 , C12N9/248 , C12Y302/01008
摘要： The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

5. 发明授权

US09926548B2 Nucleic acid constructs and host cells for producing neurospora endoglucanases 有权
公开(公告)号：US09926548B2
公开(公告)日：2018-03-27
申请号：US14917390
申请日：2014-10-23
申请人： Novozymes A/S
发明人： Weijian Lai , Lan Tang , Marc Dominique Morant , Paul Harris
IPC分类号： C12N15/56 , C12N1/21 , C12N1/15 , C12N5/10 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/79 , C12N9/42 , D06M16/00 , D06M101/04 , D06M101/06 , D06M101/40
CPC分类号： C12N9/2437 , C12Y302/01004 , D06M16/003 , D06M2101/04 , D06M2101/06 , D06M2200/35
摘要： Polypeptides having endoglucanase activity, catalytic domains, and polynucleotides encoding the polypeptides or catalytic domains. Nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.

6. 发明授权

US08815580B2 Cells producing glycoproteins having altered glycosylation patterns and method and use thereof 有权
标题翻译：产生具有改变的糖基化模式的糖蛋白的细胞及其方法和用途的细胞
公开(公告)号：US08815580B2
公开(公告)日：2014-08-26
申请号：US12737719
申请日：2009-08-10
申请人： Nico L. M. Callewaert , Karen De Pourcq , Steven Geysens , Leander Meuris
发明人： Nico L. M. Callewaert , Karen De Pourcq , Steven Geysens , Leander Meuris
IPC分类号： C12N15/79 , C12N15/87 , C12N15/56 , C12N9/24 , A61K38/00
CPC分类号： C12P21/005 , C07K2319/02 , C12N9/2402 , C12Y302/01096 , C12Y302/01113
摘要： The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells and methods are glyco-engineered yeast cells in which additionally at least one exogenous enzyme needed for complex glycosylation is present, e.g. allowing easier separation of differentially glycosylated glycoproteins.
摘要翻译：本申请涉及糖工程领域，更具体地涉及其中存在内切葡糖胺酶和糖蛋白的真核细胞。这些细胞可用于对（外源性）糖蛋白进行去糖基化或部分脱磺基化，特别是不需要添加额外的酶。还提供了用于在蛋白质生产中应用这些细胞的方法。根据一个具体方面，真核细胞和方法是糖工程酵母细胞，其中还存在复合糖基化所需的至少一种外源酶，例如，允许更容易分离差异糖基化的糖蛋白。

7. 发明授权

US08735137B2 Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization 有权
标题翻译：在解脂耶氏酵母中使用酿酒酵母SUC2基因用于蔗糖利用
公开(公告)号：US08735137B2
公开(公告)日：2014-05-27
申请号：US13296524
申请日：2011-11-15
申请人： Seung-Pyo Hong , John E. Seip , Quinn Qun Zhu
发明人： Seung-Pyo Hong , John E. Seip , Quinn Qun Zhu
IPC分类号： C12N1/15 , C12N15/56 , C12N9/26 , C12N9/60
CPC分类号： C12N9/2431 , C07K2319/02 , C12N9/60 , C12P7/64 , C12Y302/01026
摘要： Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
摘要翻译：本文公开的是转化的解脂耶氏酵母病，其包含编码具有蔗糖转化酶活性的多肽的外源多核苷酸。还公开了使用转化的解脂耶氏酵母的方法。

8. 发明授权

US08735100B2 Cellulose and ligno-cellulose active proteins 有权
标题翻译：纤维素和木质纤维素活性蛋白
公开(公告)号：US08735100B2
公开(公告)日：2014-05-27
申请号：US13257255
申请日：2010-03-17
申请人： Peter Jan Punt
发明人： Peter Jan Punt
IPC分类号： C12P19/00 , C12N15/56 , C12N15/63 , C12N9/42 , C07K14/00 , C07H21/04
CPC分类号： C12P19/14 , C12P19/02 , C12P2201/00
摘要： Methods to digest carbohydrates, especially lignocelluloses and hemicelluloses, using fungal proteins previously not recognized as having this activity are described.
摘要翻译：描述了使用以前不被认为具有该活性的真菌蛋白质来消化碳水化合物，特别是木质纤维素和半纤维素的方法。

9. 发明授权

US08623629B2 Polypeptides with xylanase activity 有权
标题翻译：具有木聚糖酶活性的多肽
公开(公告)号：US08623629B2
公开(公告)日：2014-01-07
申请号：US13141444
申请日：2009-12-23
申请人： Ole Sibbesen , Jens Frisbæk Sørensen
发明人： Ole Sibbesen , Jens Frisbæk Sørensen
IPC分类号： C12N9/24 , C12N15/56 , C07H21/04
CPC分类号： C12N9/248
摘要： Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 113, 122 or 175 in combination with one or more further amino acid modifications in position 11, 12, 13, 34, 54, 77, 81, 82, 104, 110, 113, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding to the position of Bacillus subtilis xylanase (SEQ ID NO 1).
摘要翻译：具有木聚糖酶活性的多肽被修饰以增加麸增溶和/或木聚糖酶活性。该修饰包括与位置11,12,13,34,57,78,81,82,104,110,113中的一个或多个其它氨基酸修饰相结合的位置113,122或175中的一个或多个氨基酸的修饰，118,122,141,154,159,162,164,166,175或179，其中所述位置被确定为对应于枯草芽孢杆菌木聚糖酶（SEQ ID NO 1）的位置的位置。

10. 发明申请

US20130336954A1 Staphylococcal Phage2638A Endolysin Amidase Domain Is Lytic for Staphylococcus aureus 有权
标题翻译：葡萄球菌噬菌体2638A内溶素酰胺酶域是金黄色葡萄球菌的裂液
公开(公告)号：US20130336954A1
公开(公告)日：2013-12-19
申请号：US13495536
申请日：2012-06-13
申请人： David M. Donovan , Igor V. Abaev
发明人： David M. Donovan , Igor V. Abaev
IPC分类号： C12N9/24 , C12N15/70 , A61P31/04 , A01K67/027 , A61K38/47 , C12N15/56 , C12N1/21
CPC分类号： C12N9/80 , A61K38/00 , A61L2/16 , C07K14/005 , C12N15/52 , C12N2795/10322 , C12N2795/10332 , C12N2795/10333 , C12Y305/01028
摘要： Staphylococcus aureus is notorious for developing resistance to virtually all antibiotics to which it is exposed. Staphylococcal phage 2638A endolysin is a peptidoglycan hydrolase that is lytic for S. aureus when exposed externally, making it a new antimicrobial candidate. It shares a common protein organization with over 40 other staphylococcal peptidoglycan hydrolases: a CHAP endopeptidase domain, a mid-protein amidase 2 domain and a C-terminal SH3b cell wall binding domain. It is the first phage endolysin reported with a cryptic translational start site between the CHAP and amidase domains. Deletion analysis indicates that the amidase domain confers most of the lytic activity and requires the full SH3b domain for maximal activity. It is common for one domain to demonstrate dominant activity over another; however, the phage 2638A endolysin is the first to show high amidase domain activity dominant over the N-terminal CHAP domain, an important finding for targeting novel peptidoglycan bonds.
摘要翻译：金黄色葡萄球菌对于暴露于几乎所有抗生素的抗性都是臭名昭着的。葡萄球菌噬菌体2638A细胞内溶素是一种肽聚糖水解酶，当外露时会溶解金黄色葡萄球菌，使其成为新的抗菌药物。它与40多种其他葡萄球菌肽聚糖水解酶共享一个共同的蛋白质组织：CHAP内肽酶结构域，中蛋白酰胺酶2结构域和C末端SH3b细胞壁结合结构域。这是第一个噬菌体内溶素与CHAP和酰胺酶结构域之间隐藏的翻译起始位点。缺失分析表明，酰胺酶结构域赋予大部分裂解活性，并需要完整的SH3b结构域进行最大活性。一个领域通常表现出超过另一个领域的主导地位; 然而，噬菌体2638A细胞内溶素是首先显示高N-酰基端结构域活性优于N末端CHAP结构域，这是靶向新型肽聚糖键的重要发现。

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