The invention discloses a relative transferring function of kinesin family member KIF1B, a landmark application thereof in predicting tumor patient prognosis and an application method thereof, belonging to the technical field of tumor molecular biology. The invention discloses a relative transferring function of kinesin family member KIF1B, a landmark application of KIF1B expression level in predicting tumor patient prognosis and a detection and application method based on the KIF1B expression level in predicting tumor patient prognosis. In the method, real-time quantitative RT-PCR is carried out to detect the method for KIF1B mRNA expression level; quantitative PCR amplification comprises relative quantitative PCR amplification and absolute quantitative PCR amplification; the KIF1B expression level detection can also adopt a molecular hybridization method to detect the KIF1B mRNA expression level; an immunostaining method and/ or immunoblotting method can be adopted to detect the KIF1B protein expression level; a critical value for prognosis judgment is determined according to the KIF1B gene or protein expression level in the primary cancer tissues of recurrent and metastatic positive cases and recurrent and metastatic negative cases of tumor patients; and the patient the critical value of whom is higher than the threshold value has favorable prognosis, and the patient the critical value of whom is lower than the threshold value has poor prognosis.