The invention relates to a tomato culture medium and a tomato culture method and relates to a tissue culture technique for plants. The method comprises the following steps: supplying a tomato blossoming and fruiting induction culture medium and a tomato bud multiplication culture medium; sowing processed tomato seeds in the tomato culture medium and culturing under the tomato growing condition, thereby acquiring tomato sterile seedlings; splicing the cotyledons of the tomato sterile seedlings into small laminas; inoculating the small laminas into an induction culture medium which is used for inducing the forming of the calluses and adventitious buds, and performing the induction culture, thus acquiring the adventitious buds of tomato; and culturing by inoculating the adventitious buds of tomato into the tomato blossoming and fruiting induction culture medium, thereby inducing the tomato plant to bud and fruit. By sterilely sowing the tomato seeds, cutting and taking the cotyledons andhypocotyls as explants and inducing the forming of the calluses and adventitious buds, the bud multiplication culture is performed and the tomato blossoms and fruits in a test tube. The culturing periods of tomato tissue culture and blossoming and fruiting in the test tube are short, the culture is free from being influenced by seasons, the tomato blossoming induction efficiency reaches 45%-60%, and the tomato fruiting induction efficiency reaches 30%-45%.