The invention discloses an oil-water separation chamber with multi-circuitous channels. The oil-water separation chamber comprises a separation chamber, wherein the separation chamber is provided with a cubic chamber body and is respectively provided with a mixed liquor inflow port, a lubricating oil overflow port and a cuttings liquor overflow port; the separation chamber is internally provided with multiple partition plates, the partition plates partition the separation chamber into multiple small chambers, the front several partition plates are laterally staggered and provided with gaps so that the separation chamber is of a multi-circuitous-channel structure, the last partition plate is a laterally full-closed partition plate, the bottom of the last partition plate is provided with a gap, the cuttings liquor overflow port is arranged at the top of the last small chamber, and the lubricating oil overflow port is arranged at the top of the chamber in front of the cuttings liquor overflow port. According to the invention, the oil-water separation chamber with multi-circuitous channels is simple and ingenious in structure, realizes high oil-water separation efficiency by using the multi-circuitous channels, and can be applied to an oil-water separation system of a water tank of all kinds of cutting tool machines, or a place requiring the separation of mixed liquid, and can also be applied to oil-water separation equipment, thus saving huge production cost for a customer and improving the production efficiency.