The invention discloses a method for preparing liquid utilizing methane-oxidizing bacteria to dissolve gas and a using method. The liquid comprises, by weight, 37-46% of acetic acid, 32-38% of carboxylic acid, 11-15% of animal and vegetable oil, 10-14% of glycerinum, 7-12% of top yeast extract and 5-9% of cobalt acetate, and the sum of the weight matching ratios of the components is 100%. The concept of passive prevention and treatment in the prior art is thoroughly changed, the method for preparing the liquid utilizing the methane-oxidizing bacteria to dissolve the gas and the using method belong to the field of initiative prevention and treatment, the gas is treated basically, on the other words, the gas is treated before coal is mined, the ground can be drilled while a mine is constructed, gas elimination liquid can effectively dissolve the mine gas when a coal bed is in an initial state, the mine gas density is reduced, the gas is treated within the safety range, and the personal safety of mining workers is ensured.