The invention relates to a bicycle spoke tension gauge calibration device, and belongs to the measurement and testing field. The device is composed of a force measuring machine (1), a standard spoke (2), an upper clamp (3) and a lower clamp (5). The calibration device provided by the invention solves the technical problem in calibrating the force value of a bicycle spoke tension gauge, and is simple in structure and convenient in tracing. The upper clamp and the lower clamp matched with each other are designed according to the characteristics of the force measuring machine for fixing the standard spoke, and is easy to obtain and process and convenient in use. The standard spoke as the main standard can be made to different specifications and types according to related standards; the calibration device executes quick change during work. The standard spoke can be also made to a non-standard spoke identical to the work object of the spoke tension gauge according to the specific material and dimension of the spoke so as to meet special requirements.