A bicycle damping front fork horizontal fatigue testing machine is composed of a controller, a linear motor, a force sensor, a front fork clamp, a force application clamp and a frame. The linear motor is installed on the frame, and a mover of the linear motor is equipped with the force sensor and is connected with the force application clamp through the force sensor; the front fork clamp is installed on the frame and used for installing a bicycle damping front fork test piece; and the controller is electrically connected with the linear motor, and the force sensor is electrically connected with the controller. The bicycle damping front fork horizontal fatigue testing machine of the invention can satisfy the requirements of 'bicycle damping front fork horizontal fatigue test' in a QB1881--2008 'bicycle front fork' standard, and especially, the frequency for alternate load applying is satisfied while the force application precision of the testing machine is ensured.