基本信息:
- 专利标题： 一种鳜传染性脾肾坏死病毒荧光定量PCR检测试剂盒及检测方法
- 专利标题（英）：Fluorescent quantitative PCR detection kit for infectious spleen and kidney necrosis virus and Fluorescent quantitative PCR detection method of infectious spleen and kidney necrosis virus
- 申请号：CN201410166595.8 申请日：2014-04-23
- 公开(公告)号：CN103966358A 公开(公告)日：2014-08-06
- 发明人： 林强 , 付小哲 , 李宁求 , 吴淑勤 , 石存斌
- 申请人： 中国水产科学研究院珠江水产研究所
- 申请人地址： 广东省广州市荔湾区兴渔路1号
- 专利权人： 中国水产科学研究院珠江水产研究所
- 当前专利权人： 中国水产科学研究院珠江水产研究所
- 当前专利权人地址： 广东省广州市荔湾区兴渔路1号
- 代理机构： 广州嘉权专利商标事务所有限公司
- 代理人： 郑莹
- 主分类号： C12Q1/70
- IPC分类号： C12Q1/70 ; C12Q1/68 ; C12N15/11 ; C12R1/93
The invention discloses a fluorescent quantitative PCR detection kit for infectious spleen and kidney necrosis viruses and a fluorescent quantitative PCR detection method of the infectious spleen and kidney necrosis viruses. The detection kit and the detection method are characterized in that a specific primer and a probe are designed according to the sequence of a gene conservative area of the infectious spleen and kidney necrosis virus ORF007. The detection kit and the detection method have the advantages that the fluorescent quantitative PCR technology is adopted, the detection is simple, convenient and quick, the sensitivity is high, the specificity is strong, the repeatability is good, and the detection kit and the detection method can be applicable to monitoring of the infectious spleen and kidney necrosis viruses, and can also be used for detecting the virus content and the virus titer in siniperca chuatsi.
公开/授权文献:
- CN103966358B 一种鳜传染性脾肾坏死病毒荧光定量PCR检测试剂盒及检测方法 公开/授权日：2016-04-20