
基本信息:
- 专利标题: 一种水中噬菌体分离方法
- 申请号:CN201410593832.9 申请日:2014-10-29
- 公开(公告)号:CN104312984B 公开(公告)日:2018-01-12
- 发明人: 谌志强 , 李君文 , 王新为 , 金敏 , 邱志刚 , 杨栋
- 申请人: 中国人民解放军军事医学科学院卫生学环境医学研究所
- 申请人地址: 天津市和平区大理道1号
- 专利权人: 中国人民解放军军事医学科学院卫生学环境医学研究所
- 当前专利权人: 中国人民解放军军事医学科学院卫生学环境医学研究所
- 当前专利权人地址: 天津市和平区大理道1号
- 代理机构: 北京高沃律师事务所
- 代理人: 王加贵
- 主分类号: C12N7/02
- IPC分类号: C12N7/02 ; C12R1/92
The invention discloses a method for separating phage in water. The method comprises the following steps: (1) preparing positive charge silica gel particles; (2) preparing eluent; and (3) separating the phage. According to the method disclosed by the invention, the phage in various water environments such as running water, surface water and sewage can be efficiently separated, the separation efficiency is high, and the separation efficiency on the phage of extremely low concentration (100PFU/100L) in the water is 70 percent. The method has the advantages of simplicity and convenience in operation, rapidness and low price. Therefore, the method has the advantages that the speed of separating novel phages from the water environment is increased, and assistance is provided for application of the phages.
公开/授权文献:
- CN104312984A 一种水中噬菌体分离方法 公开/授权日:2015-01-28