基本信息:
- 专利标题： 一种用于检测阿特拉津的金标二抗信号放大SRP免疫传感方法
- 专利标题（英）：Gold-labeled secondary antibody signal amplified SRP immunosensing method for detection of atrazine
- 申请号：CN201310450946.3 申请日：2013-09-27
- 公开(公告)号：CN104515752A 公开(公告)日：2015-04-15
- 发明人： 彭媛 , 高志贤 , 宁保安 , 白家磊 , 孙思明 , 柳明
- 申请人： 中国人民解放军军事医学科学院卫生学环境医学研究所
- 申请人地址： 天津市和平区大理道1号
- 专利权人： 中国人民解放军军事医学科学院卫生学环境医学研究所
- 当前专利权人： 中国人民解放军军事医学科学院卫生学环境医学研究所
- 当前专利权人地址： 天津市和平区大理道1号
- 主分类号： G01N21/552
- IPC分类号： G01N21/552 ; G01N33/577 ; G01N33/532
The invention discloses a gold-labeled secondary antibody signal amplified SRP (surface plasmon resonance) immunosensing method for detection of atrazine. The method adopts antigen-antibody immunoreaction as the basic principle, an antigen or antibody is taken as a sensor sensitive recognition element, an indirect competitive technique and an SPR sensing technology are combined to detect the herbicide atrazine, an atrazine complete antigen (AT-OVA) is fixed on the surface of an SPR chip through a chemical bond, competitive binding with the antibody is carried out on the atrazine small molecules in a to-be-detected solution, a nanogold-labeled secondary antibody is added to perform signal amplification, the detection sensitivity is enhanced, the detection range is improved, and the signal amplified indirect competitive inhibition method is established. The lowest detection limit of the method is 0.72ng/mL, IC50 is 15.70ng/mL, and the detection range is 2.18-629.06ng/mL.
公开/授权文献:
- CN104515752B 一种用于检测阿特拉津的金标二抗信号放大SRP免疫传感方法 公开/授权日：2017-07-18
IPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N21/00 | 利用光学手段，即利用红外光、可见光或紫外光来测试或分析材料 |
| --------G01N21/01 | .便于进行光学测试的装置或仪器 |
| ----------G01N21/21 | ..影响偏振的性质 |
| ------------G01N21/552 | ...衰减全反射 |