The invention discloses a guide rail fixation device for an automobile window-lifting guide rail tester. The guide rail fixation device for the automobile window-lifting guide rail tester comprises a rotary pressing-down device, a first fixation block, an induction pressing device, a second fixation block and a pressing device. The upper ends of the first fixation block and the second fixation block are provided with a guide rail; the front end of the guide rail is fixed to a rotary fixation block of the rotary pressing-down device; the middle portion of the guide rail is fixed to an induction fixation mould of the induction pressing device; and the back end of the guide rail is fixed to a pressing fixation block of the pressing device. The rotary pressing-down device comprises a rotary baffle block, a rotary pressing-down cylinder, a first connection block, a second connection block, a connection column and a press head. A piston rod of the rotary pressing-down cylinder is inserted to the first connection block; the first connection block is connected with the second connection block through a pin; the connection column is inserted to the second connection block; and the lower end of the connection column is equipped with the press head. Through the mode above, the guide rail fixation device can automatically clamp the guide rail instead of workers, thereby saving labor force and preventing resource waste.