The invention discloses a window lifting guide rail test device of an automobile window lifting guide rail test machine. The window lifting guide rail test device of the automobile window lifting guide rail test machine comprises a detection platform, a connection board, a platform support, a left side guide rail detection mechanism, a right side guide rail detection mechanism and an installing hole detection mechanism. An upper end of the platform support is provided with a detection platform. Two ends of an upper plane of the detection platform are provided with the left side guide rail detection mechanism and the right side guide rail detection mechanism. A lower plane of the detection platform is provided with the installing hole detection mechanism. An intermediate portion of the upper plane of the detection platform is provided with the connection board. An upper plane of the connection board is provided with two handles. The connection board is fixed to the detection platform through screwing in a screw The right side guide rail detection mechanism comprises a guide rail fixing device and a radian detection apparatus. A right side of the guide rail fixing device is provided with the radian detection apparatus. Through the above mode, the device can be used to replace a worker to detect an automobile window lifting guide rail so that a labor force is saved and production efficiency is increased.