基本信息:
- 专利标题： 一种亚铁氧化酶的速率测定法
- 专利标题（英）：Determination method of rate of ferrous oxidase
- 申请号：CN201510772601.9 申请日：2015-11-13
- 公开(公告)号：CN105466869A 公开(公告)日：2016-04-06
- 发明人： 李彬先 , 王丹峰 , 关晓辉 , 王守岗 , 马靖 , 王玉辉 , 董理
- 申请人： 李彬先
- 申请人地址： 吉林省吉林市船营区松北二区4-5-56号
- 专利权人： 李彬先
- 当前专利权人： 李彬先
- 当前专利权人地址： 吉林省吉林市船营区松北二区4-5-56号
- 主分类号： G01N21/31
- IPC分类号： G01N21/31
The present invention discloses a determination method of rate of ferrous oxidase. The method is as below: incubating a serum sample in a known amount of ferrous ions acetate buffer (0.45mol / L, pH of 5.8); oxidizing the ferrous ions into ferric ions under the effect of the oxidase; incubating for a certain period of time, adding a chromogen ferrozine to form a colored complex; measuring the content of unoxidized ferrous ions at a wavelength of 510m; and determining the concentration of ferrous oxidase by using the difference between the concentrations of ferrous ions before and after the enzymatic reaction to represent the number of oxidized ferrous ions. The determination method has short reaction time and coloration stability, meets the requirements for manual operation of an automatic biochemical analyzer and a spectrophotometer, and also improves the detection precision.
IPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N21/00 | 利用光学手段，即利用红外光、可见光或紫外光来测试或分析材料 |
| --------G01N21/01 | .便于进行光学测试的装置或仪器 |
| ----------G01N21/21 | ..影响偏振的性质 |
| ------------G01N21/31 | ...测试材料在特定元素或分子的特征波长下的相对效应，例如原子吸收光谱术 |