The invention relates to a daily optimization regulation control method for water conveying systems of step pump stations. The method comprises the steps of establishing a hydraulic simulation model and calculating water head loss; performing optimization distribution on flow of a unit in each pump station; performing optimization distribution on lift of each pump station in the step pump stations; and performing optimization distribution on water diversion volume at each time period in a day. According to the method, the optimization regulation control problem of the water conveying systems of the multi-constraint and multi-target step pump stations is taken as a research object, storage ponds, channels and the step pump stations are comprehensively considered, the hydraulic simulation model and a large system decomposition-coordination method are synthesized, and a daily optimization regulation control mode for a water diversion project is established, so that scientific bases and technical support can be provided for actual regulation control. Compared with the prior art, the method has the characteristics that the constraints such as the storage constraints of the storage ponds, the water head loss of the channels, the total lift of the step pump stations and the like are provided, so that the constraint conditions of project regulation control can better meet the requirements of the water conveying systems in the water diversion project; and the economic benefits of the water conveying systems are maximized by taking the minimum sum of abandoned water and the total electricity fee of the step pump stations as a target function.