The invention relates to a high dye uptake disperse dye composition and a preparation method and application thereof. The disperse dye composition is prepared from a compound shown in the formula I with the content accounting for 5-25% of the total mass of the dye composition, a compound shown in the formula II with the content accounting for 5-25% of the total mass of the dye composition, a compound shown in the formula III with the content accounting for 0.5-10% of the total mass of the dye composition, a compound shown in the formula IV with the content accounting for 0.5-8% of the total mass of the dye composition, and the balance additive. The preparation method comprises the steps that the compounds in the formulas I, II, III and IV and the additive are mixed to be uniform and are ground and subjected to spray drying so as to obtain the high dye uptake disperse dye composition. The disperse dye composition is applied to dyeing of hydrophobic fiber or fiber blends. The disperse dye composition has the good soaping fastness, perspiration fastness, sublimation fastness and whiteness pitching fastness. The preparation method is simple in technology, low in cost and good in application prospect.