基本信息:
- 专利标题： 一种促进人表皮细胞生长因子在大肠杆菌高效功能性表达的方法
- 专利标题（英）：Method for promoting high-efficiency functional expression of human epidermal cell growth factor in Escherichia coli
- 申请号：CN201610586211.7 申请日：2016-07-22
- 公开(公告)号：CN106244610A 公开(公告)日：2016-12-21
- 发明人： 王菊芳 , 马毅 , 李杉 , 吴少敏 , 余结莹
- 申请人： 华南理工大学
- 申请人地址： 广东省广州市天河区五山路381号
- 专利权人： 华南理工大学
- 当前专利权人： 华南理工大学
- 当前专利权人地址： 广东省广州市天河区五山路381号
- 代理机构： 广州市华学知识产权代理有限公司
- 代理人： 崔红丽
- 主分类号： C12N15/70
- IPC分类号： C12N15/70 ; C07K14/485 ; C07K1/36 ; C07K1/34 ; C07K1/22
The invention discloses a method for promoting the high-efficiency functional expression of human epidermal cell growth factor in Escherichia coli. According to the method, through the construction of expression vector pET21a-HEGF-Mxe-SUMO, high-efficiency soluble expression of hEGF protein without any exogenous amino acids in Escherichia coli BL21 (DE3) can be achieved. An effective idea and method is provided for promoting soluble expression of the exogenous protein in Escherichia coli. By fusion of a SUMO tag to the C end of a target protein, the functional expression efficiency of the fusion protein can be effectively improved, self-cleavable intein is further added into the fusion protein, and the intein and SUMO protein in the fusion protein can be cut in reducing environment so as to obtain the biological-active human epidermal cell growth factor without any exogenous amino acid sequences. The method provides a theoretical basis and new design ideas for the efficient production of complex eukaryotic biological proteins in prokaryotic expression system.
公开/授权文献:
- CN106244610B 一种促进人表皮细胞生长因子在大肠杆菌高效功能性表达的方法 公开/授权日：2019-07-16