The invention relates to a preparation process of watermelon red wine. The preparation process is characterized by comprising the following steps: extracting watermelon juice from watermelon, red globe grape juice from red globe grapes, red sour jujube juice from red sour jujubes, and red raspberry juice from red raspberries, carrying out anti-oxidation treatment, so that the original red flavor is protected, adding white sticky rice into residues obtained by filtering fruit and vegetable juice, and carrying out fermentation by adopting a common fermentation process to prepare a wine base, thus obtaining a crude filtrate; mixing the fruit and vegetable juice and the crude filtrate of the wine base, adjusting the alcohol content and sweetness, carrying out ageing for 4-6 months, finally, carrying out clarifying, filtering and sterilizing, carrying out canning, and carrying out packaging, thus obtaining the beauty and health care watermelon red wine.