The invention provides an automobile teacup box fatigue detection device which can solve the technical problems that the existing fatigue detection device is complex in structure, complicated in operation and high in cost. The technical scheme is that the automobile teacup box fatigue detection device comprises a baseplate, a fixing mechanism, an opening mechanism, a covering mechanism and a counting sensor. The fixing mechanism comprises a teacup base which is convexly provided with multiple elastic connecting arms. The multiple elastic connecting arms form a cavity for accommodating a teacup box through enclosing and can elastically clamp the teacup box. The two sides of the teacup base are respectively provided with a locking and positioning plate which is provided with a locking member used for fixing a rotating buckling plate. The opening mechanism comprises an air cylinder which is fixed on an air cylinder base, and the piston end part of the air cylinder is provided with an elastic bump head. The covering mechanism comprises a pressing rod which is connected with the output shaft of a stepping motor through a swing arm and arranged to be parallel to the output shaft of the stepping motor. The counting sensor is fixed on a sensor supporting base.