The invention discloses meconopsis grandis endophytic fungi DH24 and application thereof to fermentation production of pyrrocidines compounds. Meconopsis grandis endophytic fungi DH24 strains were preserved in Microbial Strain Preservation Center of Guangdong in September 23, 2016, and are assigned the accession number GDMCC No: 60081, and the preservation address is Floor 5, Building No. 59, Xianlie Middle Road No. 100 courtyard, Guangzhou. The Tibet medicine endophytic fungi DH24 can generate pyrrocidines compounds which are of a unique structure; and through testing, the compounds have activities such as antitumor effect in vitro and antifungal in vitro. Besides, the meconopsis grandis endophytic fungi DH24 is simple in fermentation process and short in cycle, the cost for producing the active compounds is low, the meconopsis grandis endophytic fungi DH24 saves energy, is environmentally friendly and is an ideal carrier for developing novel natural medicines, production is amplified easily, and the meconopsis grandis endophytic fungi DH24 is suitable for large-scale production and good in application prospect.