The invention discloses an eye washing liquid formula and a preparation method thereof and belongs to the field of medicines. The formula comprises the following components in parts by weight: 0.01 to0.1 percent of hyaluronic acid, 0.01 to 0.09 percent of tea polyphenol, 0.01 to 0.09 percent of lutein, 0.01 to 0.09 percent of pearl powder and 0.01 to 0.1 percent of menthol; if eye washing liquidis not packaged in vacuum, the ratio of an added chemical or plant preservative is 0.02 to 0.05 percent and the residual ratio is purified water. The eye washing liquid prepared by the method has moderate performance and is moist and can be used for effectively cleaning and moistening eyes, alleviating eye fatigue symptoms including dry eyes, swelling, pains and itching and the like and rapidly relaxing the eyes; the eye washing liquid has effects of preventing and controlling myopia, and alleviating the eye fatigue symptoms to prevent eye diseases; the formula has a trophic action and can beused for protecting eyesight.