The invention relates to a preparation technology of benzisothiazolinone temperature sensitive type in situ gel for pig wombs. The preparation technology comprises the following steps: a first step, taking 0.5-15 grams of benzisothiazolinone, pouring the taken benzisothiazolinone into 100 milliliters of purified water, performing slow heating to 80 DEG C, adding 0.4-10 grams of a solubilizer, stirring until a complete dissolving state is achieved, performing heat-preservation stirring for 30 minutes to 1 hour to obtain a solution, adding water until the volume of the solution and the water is200 milliliters, and performing uniform stirring to obtain a sovereign drug solution; a second step, respectively and precisely weighing 120-230 grams of gel substrates A, 1-20 grams of gel substratesB, enabling the weighed gel substrates and the weighed gel substrates B to dissolve in 600 milliliters of distilled water to obtain a mixture, then placing the mixture in a refrigerator of 4 DEG C, and standing in the refrigerator of 4 DEG C for 24 hours so as to obtain a clear and bright solution as a gel substrate; a third step, adding an antioxidant to 100 milliliters of purified water, and performing stirring for dissolving the antioxidant so as to obtain an antioxidant solution; a fourth step, adding the antioxidant solution to the sovereign drug solution, performing uniform stirring toobtain a new solution, adding water until the volume of the new solution and the water is 1L, and performing uniform stirring and mixing so as to obtain a mixed solution; and a fifth step, filtering the prepared mixed solution, charging nitrogen gas, and performing encapsulation in brown bottles of 50-500 milliliters. The preparation of the benzisothiazolinone temperature sensitive type in situ gel disclosed by the invention is high in safety, and can effectively treat endometritis of pigs.