基本信息:
- 专利标题： 一种藏红花来源的CCD2突变体及其编码序列和应用以及生产藏红花酸的重组酵母菌株
- 专利标题（英）：Saffron crocus sourced CCD2 mutant, coding sequence and application thereof, as well as recombinant yeast strain for producing crocetin
- 申请号：CN201910243448.9 申请日：2019-03-28
- 公开(公告)号：CN109810958A 公开(公告)日：2019-05-28
- 发明人： 元英进 , 梁楠 , 姚明东 , 王颖 , 肖文海
- 申请人： 天津大学
- 申请人地址： 天津市南开区卫津路92号
- 专利权人： 天津大学
- 当前专利权人： 天津大学
- 当前专利权人地址： 天津市南开区卫津路92号
- 代理机构： 北京集佳知识产权代理有限公司
- 代理人： 刘猛; 赵青朵
- 主分类号： C12N9/02
- IPC分类号： C12N9/02 ; C12N15/53 ; C12N15/81 ; C12N1/19 ; C12P11/00 ; C12R1/865
The invention relates to the technical field of genetic engineering, and discloses a saffron crocus sourced CCD2 mutant, a coding sequence and application thereof, as well as a recombinant yeast strain for producing crocetin. The CCD2 mutant is provided with one or two or above of V120F, Y190K/A, R192V/F, E211A, E212A, T290V, K320A and S323F/A/T locus mutations. A rate-limiting enzyme CCD2 in thesynthesis route of the crocetin is transformed, the crocetin is directly synthesized from beta-carotene by widening the substrate spectrum of the CCD2, and the existing synthesis route of the crocetinis simplified and reconstructed, so that the problem that the total yield of multiple reactions is generally low is solved; and meanwhile, the efficiency of the CCD2 catalyzing an original substratezeaxanthin to synthesize the crocetin is improved, and the yeast strain of the high-yield crocetin is further obtained.
公开/授权文献:
- CN109810958B 一种藏红花来源的CCD2突变体及其编码序列和应用以及生产藏红花酸的重组酵母菌株 公开/授权日：2020-10-02