The invention belongs to intelligent cleaning, and particularly relates to a test tube cleaning device. The test tube cleaning device comprises a cleaning table, a supporting rod and a cover plate, wherein the supporting rod is vertically and fixedly arranged in the center of the top of the cleaning table, and the cover plate vertically sleeves the supporting rod; a plurality of overflow holes areformed in the top of the cleaning table, circular plugs are concentrically embedded in the overflow holes, an annular water permeable hole is formed between each circular plug and each overflow hole,hollow supporting rods perpendicular to the circle center of the circular plugs are arranged on the circular plugs, a horizontal separation plate is further arranged in the cleaning table, a plurality of water permeation holes are formed in the horizontal separation plate, the lower ends of the hollow supporting rods are fixedly arranged on the horizontal partition plate, and the lower ends of the hollow supporting rods are connected with a water pump through a guide pipes, a water storage bin is arranged below the horizontal separation plate, and the water pump is arranged at the bottom of the water storage bin; and the top ends of the hollow supporting rods are connected with cylindrical cleaning heads, and a plurality of water spraying holes are formed in the cleaning heads.