The invention discloses a grid used in a grid-controlled pulse traveling wave tube electron gun and a manufacturing process thereof. The grid includes a grid substrate made of molybdenum, a gradient functional layer formed on the grid substrate and a composite anti-grid electron emission film formed on the gradient functional layer. The gradient functional layer is prepared by Nb or Ta. The composite anti-grid electron emission film is prepared by using a high-melting point material having a work function of more than 4 eV. The manufacturing process includes the following steps: (1) performinghigh temperature vacuum degassing on the formed grid substrate of a molybdenum material at 700-1000 DEG C; (2) preparing the gradient functional layer on the grid substrate by using ion implantationand vacuum deposition coating; (3) preparing the composite anti-grid electron emission film on the gradient functional layer by using the ion implantation and vacuum deposition coating; and (4) performing high temperature heat treatment on the prepared grid. The grid of the invention can suppress grid electron emission of a grid-controlled pulse traveling wave tube and improve the working reliability and service life of the grid-controlled pulse traveling wave tube.