基本信息:
- 专利标题： 一种高浓度蒎烯耐受性产蒎烯工程菌的构建方法
- 专利标题（英）：Construction method of high-concentration pinene tolerance pinene producing engineering bacteria
- 申请号：CN201911049349.3 申请日：2019-10-31
- 公开(公告)号：CN110656056A 公开(公告)日：2020-01-07
- 发明人： 诸葛斌 , 马腾飞 , 宗红 , 陆信曜 , 任全路
- 申请人： 江南大学
- 申请人地址： 江苏省无锡市滨湖区蠡湖大道1800号张勇
- 专利权人： 江南大学
- 当前专利权人： 江南大学
- 当前专利权人地址： 江苏省无锡市滨湖区蠡湖大道1800号张勇
- 代理机构： 哈尔滨市阳光惠远知识产权代理有限公司
- 代理人： 张勇
- 主分类号： C12N1/19
- IPC分类号： C12N1/19 ; C12P5/00 ; C12R1/72
The invention discloses a construction method of a high-concentration pinene tolerance pinene producing engineering bacteria, and belongs to the technical field of microorganisms. The construction method comprises the steps of selecting hyperosmosis resisting candida glycerinogenes (Candida glycerinogenes) CCTCC M 93018 transformation glucose, and performing fermentation for producing pinene. Pinene tolerance by yeast can achieve 1g/L, growth inhibition of host production strains by the pinene can be effectively overcome, and the yield of the pinene achieves 0.8mg/L. The preparation method hasthe characteristics of being simple to operate, high in transformation efficiency, low in production cost, broad in industrial application prospect and the like.
公开/授权文献:
- CN110656056B 一种高浓度蒎烯耐受性产蒎烯工程菌的构建方法 公开/授权日：2021-07-27