The invention discloses an air-water pulse pipeline cleaning device with an ozone cleaning function and a disinfection function. The device comprises an air compressor, an ozone generator, an air-water mixer, an air-water pulse generator and a controller, during operation, one part of compressed air of the air compressor enters the air-water pulse generator, and the other one part of the compressed air enters the ozone generator, the ozone generator produces ozone gas, the ozone gas and external water source enter the air-water mixer, after mixing, ozone water with a certain concentration is conveyed into the air-water pulse generator, the air-water pulse generator works so as to enable the compressed air and the ozone water to enter a to-be-cleaned pipeline through an outlet of the air-water pulse generator according to a certain output frequency, air-water pulse waves mixed by air and water are generated, water hammer and microwave oscillation are formed, ozone can remove rust in anoxidizing mode and perform sterilization and disinfection, the purpose of disinfecting and sterilizing the pipeline is achieved while dirt in the pipeline is cleared, the working efficiency of the cleaning device is improved, and the applicability of the cleaning device is improved.