会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明专利
    • Method for preparing tnfr-fc fusion protein containing target content of impurities
    • IL253218B
    • 2022-08-01
    • IL25321817
    • 2017-06-28
    • LG CHEMICAL LTDSANG WOO PARKHWA YOUNG LEESOON WOONG CHOICHUL HO JUNG
    • SANG WOO PARKHWA YOUNG LEESOON WOONG CHOICHUL HO JUNG
    • C07K14/705C07K16/00C07K19/00
    • The present invention relates to a method for preparing a TNFR-Fc fusion protein mixture comprising a target content of hydrophobic chromatogram peak 3, and to a method for adjusting a content of hydrophobic chromatogram peak 3. Specifically, the present invention relates to (a) a method for preparing a TNFR-Fc fusion protein mixture using a hydrophobic interaction chromatograph medium containing an aromatic functional group, which is pre-equilibrated with an equilibration buffer comprising sodium chloride or ammonium sulfate, and a sample comprising a TNFR-Fc fusion protein mixture liquid produced from mammalian cells, and to a method for adjusting the content of hydrophobic chromatogram peak 3 by hydrophobic chromatography using an equilibration buffer containing a predetermined concentration of sodium chloride or ammonium sulfate. The present invention can provide a method for producing a TNFR-Fc fusion protein by, in the production of the TNFR-Fc fusion protein using hydrophobic chromatography, performing hydrophobic chromatography through equilibration using an equilibration buffer containing a predetermined concentration of sodium chloride or ammonium sulfate, thereby adjusting the content of hydrophobic chromatogram peak 3 to contain a target content of hydrophobic chromatogram peak 3. Therefore, the method presented above can be favorably used in the manufacturing of biological medicines comprising a recombinant protein, such as etanercept produced from animal cell culture through genetic recombinant technology.
    • 2. 发明专利
    • Method for preparing tnfr-fc fusion protein containing target content of impurities
    • IL253218D0
    • 2017-08-31
    • IL25321817
    • 2017-06-28
    • LG CHEMICAL LTDSANG WOO PARKHWA YOUNG LEESOON WOONG CHOICHUL HO JUNG
    • SANG WOO PARKHWA YOUNG LEESOON WOONG CHOICHUL HO JUNG
    • C07K20060101
    • The present invention relates to a method for preparing a TNFR-Fc fusion protein mixture comprising a target content of hydrophobic chromatogram peak 3, and to a method for adjusting a content of hydrophobic chromatogram peak 3. Specifically, the present invention relates to (a) a method for preparing a TNFR-Fc fusion protein mixture using a hydrophobic interaction chromatograph medium containing an aromatic functional group, which is pre-equilibrated with an equilibration buffer comprising sodium chloride or ammonium sulfate, and a sample comprising a TNFR-Fc fusion protein mixture liquid produced from mammalian cells, and to a method for adjusting the content of hydrophobic chromatogram peak 3 by hydrophobic chromatography using an equilibration buffer containing a predetermined concentration of sodium chloride or ammonium sulfate. The present invention can provide a method for producing a TNFR-Fc fusion protein by, in the production of the TNFR-Fc fusion protein using hydrophobic chromatography, performing hydrophobic chromatography through equilibration using an equilibration buffer containing a predetermined concentration of sodium chloride or ammonium sulfate, thereby adjusting the content of hydrophobic chromatogram peak 3 to contain a target content of hydrophobic chromatogram peak 3. Therefore, the method presented above can be favorably used in the manufacturing of biological medicines comprising a recombinant protein, such as etanercept produced from animal cell culture through genetic recombinant technology.