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1. 发明申请

US20080248479A1 Methods for Isolating and Characterizing Endogenous mRNA-Protein (mRNP) Complexes 有权
标题翻译：分离和表征内源性mRNA蛋白（mRNP）复合物的方法
公开(公告)号：US20080248479A1
公开(公告)日：2008-10-09
申请号：US12049862
申请日：2008-03-17
申请人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
发明人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
IPC分类号： C12Q1/68
CPC分类号： G01N33/5008 , C07H21/02 , C12Q1/6804 , C12Q1/6809 , C12Q2600/158 , G01N33/5023 , G01N33/5308 , G01N33/68 , G01N33/6803 , G01N33/6845 , C12Q2522/101
摘要： Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained.
摘要翻译：通过使生物样品与至少一种特异性结合mRNP复合物的至少一种组分的配体接触，在体内分配细胞mRNA-蛋白（mRNP）复合物。合适的生物样品包含至少一种mRNA-蛋白（mRNP）复合物，并且包括细胞培养物，细胞提取物和包括肿瘤组织的整个组织。配体包括特异性结合存在于mRNP复合物中的RNA结合或RNA相关蛋白的抗体。通过将配体与对配体特异性的结合分子结合来分离mRNP复合物，其中结合分子连接到固体支持物上。通过从固体支持物上除去mRNP复合物来收集mRNP复合物。在收集mRNP复合物后，可以鉴定和鉴定复合物内结合的mRNA。因此可以对细胞的总mRNA群体的亚群进行表征，并获得所获得的细胞的基因表达谱。

2. 发明申请

US20080206763A1 METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES 审中-公开
公开(公告)号：US20080206763A1
公开(公告)日：2008-08-28
申请号：US11924440
申请日：2007-10-25
申请人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
发明人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
IPC分类号： C12Q1/68
CPC分类号： G01N33/5008 , C07H21/02 , C12Q1/6804 , C12Q1/6809 , C12Q2600/158 , G01N33/5023 , G01N33/5308 , G01N33/68 , G01N33/6803 , G01N33/6845 , C12Q2522/101
摘要： Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained.

3. 发明授权

US5561222A RNA-binding proteins useful for the control of cellular genetic processing and expression 失效
标题翻译：可用于控制细胞遗传处理和表达的RNA结合蛋白
公开(公告)号：US5561222A
公开(公告)日：1996-10-01
申请号：US173941
申请日：1993-12-27
申请人： Jack D. Keene , Charles C. Query , Rex O. Bentley
发明人： Jack D. Keene , Charles C. Query , Rex O. Bentley
IPC分类号： C07K14/47 , C12N15/00 , C07K14/00
CPC分类号： C07K14/47
摘要： Ribonucleoproteins and DNA-binding proteins related to the amino acid sequence corresponding at least to residues 92-202 and up to about residues 1 to about 240 of sequence (III), or of a modification of such sequences (III) having at least 35% homology with sequence (III) are disclosed.
摘要翻译：与至少对应于序列（III）的残基92-202和至多约残基1至约240的氨基酸序列相关的核糖核蛋白和DNA结合蛋白，或具有至少35％的序列（III）的修饰，公开了与序列（III）的同源性。

4. 发明授权

US5444149A Methods and compositions useful in the recognition, binding and expression of ribonucleic acids involved in cell growth, neoplasia and immunoregulation 失效
标题翻译：涉及细胞生长，瘤形成和免疫调节的核糖核酸的识别，结合和表达的方法和组合物
公开(公告)号：US5444149A
公开(公告)日：1995-08-22
申请号：US881075
申请日：1992-05-11
申请人： Jack D. Keene , Peter H. King
发明人： Jack D. Keene , Peter H. King
IPC分类号： C07K14/00 , C07K14/47
CPC分类号： C07K14/00 , C07K14/4702 , C12N2799/027
摘要： A peptide, Hel-N1 (SEQ ID NO: 2), which can bind to a 3'-untranslated mRNA sequence (which encompasses the "instability sequence") that is uniquely present in the messenger RNAs that encode oncoproteins and lymphokines, and mediates the specific destruction of the messenger RNAs, is described. Full-length Hel-N1 is capable of suppressing cell growth and causing cellular differentiation. Hel-N1 (SEQ ID NO: 2) possess three RNA recognition motifs. One of these forms an RNA-binding domain which, when transfected alone into cells, causes them to undergo rapid growth.
摘要翻译：可以结合编码癌蛋白和淋巴因子的信使RNA中唯一存在的3'非翻译的mRNA序列（其包括“不稳定序列”）的肽Hel-N1（SEQ ID NO：2）和介导描述了信使RNA的特异性破坏。全长Hel-N1能够抑制细胞生长并引起细胞分化。 Hel-N1（SEQ ID NO：2）具有三个RNA识别基序。其中一种形成RNA结合结构域，当单独将其转染入细胞时，其使其经历快速生长。

5. 发明授权

US4751181A Methods and compositions useful in the diagnosis and treatment of autoimmune diseases 失效
标题翻译：用于诊断和治疗自身免疫性疾病的方法和组合物
公开(公告)号：US4751181A
公开(公告)日：1988-06-14
申请号：US687908
申请日：1984-12-31
申请人： Jack D. Keene
发明人： Jack D. Keene
IPC分类号： G01N33/531 , A61K38/00 , A61K38/17 , A61K39/00 , C07K14/00 , C07K14/47 , C12N15/00 , C12N15/09 , C12N15/11 , C12P21/00 , C12R1/19 , G01N33/564 , C12P21/02 , C12N1/00 , C12Q1/68
CPC分类号： C07K14/4713 , A61K39/0008 , C12N15/11 , G01N33/564 , G01N2800/24 , Y10S436/811
摘要： A method for producing a protein antigen which is reactive with an autoantibody associated with an autoimmune disease in a host, which comprises introducing genetic information from a cross-reactive donor gene library, into plural cells thereby producing transformed cells; selecting a producer cell which expresses said antigen by detecting a binding reaction between said autoantibody obtained from said host and a protein antigen expressed by a producer cell of said transformed cells which contains a gene coding for said protein antigen, thereby identifying a cloned DNA segment from said donor which can be utilized in the production of said protein, is disclosed along with biochemical reagents and products associated with this invention.
摘要翻译：一种产生与宿主中与自身免疫疾病相关的自身抗体反应的蛋白质抗原的方法，其包括将来自交叉反应供体基因文库的遗传信息引入多个细胞，由此产生转化细胞; 通过检测从所述宿主获得的所述自身抗体与包含编码所述蛋白质抗原的基因的所述转化细胞的生成细胞所表达的蛋白质抗原之间的结合反应来选择表达所述抗原的生产细胞，从而鉴定克隆的DNA片段可用于生产所述蛋白质的所述供体与本发明相关的生物化学试剂和产品一起公开。

6. 发明授权

US08815517B2 Methods for identifying functionally related genes and drug targets 有权
标题翻译：识别功能相关基因和药物靶标的方法
公开(公告)号：US08815517B2
公开(公告)日：2014-08-26
申请号：US10309788
申请日：2002-12-04
申请人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson , William C. Phelps
发明人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson , William C. Phelps
IPC分类号： C12Q1/68 , C12N15/09 , C07K17/04 , C07K19/00 , C12N15/07 , C12N15/11
CPC分类号： C07H21/02 , C12Q1/68 , C12Q1/6804 , C12Q1/6809 , C12Q2600/158 , G01N33/5008 , G01N33/5014 , G01N33/502 , G01N33/5308 , G01N33/68 , G01N33/6845 , G01N33/6848 , C12Q2522/101
摘要： The identification and evaluation of mRNA and protein targets associated with mRNP complexes and implicated in the expression of proteins involved in common physiological pathways is described. Effective targets are useful for treating a disease, condition or disorder associated with the physiological pathway.
摘要翻译：描述了与mRNP复合物相关的mRNA和蛋白质靶标的鉴定和评估，并涉及涉及常见生理学途径的蛋白质的表达。有效的靶标可用于治疗与生理途径相关的疾病，病症或障碍。

7. 发明申请

US20090263790A1 Methods for Identifying Functionally Related Genes and Drug Targets 审中-公开
标题翻译：识别功能相关基因和药物靶标的方法
公开(公告)号：US20090263790A1
公开(公告)日：2009-10-22
申请号：US11926286
申请日：2007-10-29
申请人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson , William C. Phelps
发明人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson , William C. Phelps
IPC分类号： C12Q1/68
CPC分类号： C07H21/02 , C12Q1/68 , C12Q1/6804 , C12Q1/6809 , C12Q2600/158 , G01N33/5008 , G01N33/5014 , G01N33/502 , G01N33/5308 , G01N33/68 , G01N33/6845 , G01N33/6848 , C12Q2522/101
摘要： The identification and evaluation of mRNA and protein targets associated with mRNP complexes and implicated in the expression of proteins involved in common physiological pathways is described. Effective targets are useful for treating a disease, condition or disorder associated with the physiological pathway.
摘要翻译：描述了与mRNP复合物相关的mRNA和蛋白质靶标的鉴定和评估，并涉及涉及常见生理学途径的蛋白质的表达。有效的靶标可用于治疗与生理途径相关的疾病，病症或障碍。

8. 发明授权

US07504210B2 Methods for isolating and characterizing endogenous mRNA-protein (mRNP) complexes 有权
公开(公告)号：US07504210B2
公开(公告)日：2009-03-17
申请号：US10629453
申请日：2003-07-29
申请人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
发明人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/567 , C12P19/34 , C12N15/00 , C07H21/02
CPC分类号： G01N33/5008 , C07H21/02 , C12Q1/6804 , C12Q1/6809 , C12Q2600/158 , G01N33/5023 , G01N33/5308 , G01N33/68 , G01N33/6803 , G01N33/6845 , C12Q2522/101
摘要： Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained.

9. 发明申请

US20080254461A1 METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES 审中-公开
公开(公告)号：US20080254461A1
公开(公告)日：2008-10-16
申请号：US11926091
申请日：2007-10-28
申请人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
发明人： Jack D. Keene , Scott A. Tenenbaum , Craig C. Carson
IPC分类号： C12Q1/68
CPC分类号： G01N33/5008 , C07H21/02 , C12Q1/6804 , C12Q1/6809 , C12Q2600/158 , G01N33/5023 , G01N33/5308 , G01N33/68 , G01N33/6803 , G01N33/6845 , C12Q2522/101
摘要： Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained.

10. 发明授权

US07432052B2 Method and identification of downstream mRNA ligands to FMRP and their role in fragile X syndrome and associated disorders 失效
标题翻译： FMRP下游mRNA配体的方法和鉴定及其在脆性X综合征及相关疾病中的作用
公开(公告)号：US07432052B2
公开(公告)日：2008-10-07
申请号：US10495728
申请日：2002-11-15
申请人： Stephen T. Warren , Victoria Brown-Kennerly , Peng Jin , Stephanie Ceman , Robert B. Darnell , Jennifer C. Darnell , Jack D. Keene , Scott A. Tenenbaum
发明人： Stephen T. Warren , Victoria Brown-Kennerly , Peng Jin , Stephanie Ceman , Robert B. Darnell , Jennifer C. Darnell , Jack D. Keene , Scott A. Tenenbaum
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/158
摘要： Compositions and methods for identifying and/or modulating RNA transcripts and/or genes involved in fragile X syndrome and other associated disorders are provided. In particular, RNA targets for fragile X mental retardation protein (FMRP) have been identified by a novel monoclonal antibody to FMRP and a consensus sequence for the RNA binding region has been identified. Arrays for identifying compounds, proteins, nucleotides, and the like that modulate the RNA targets or associated genes are provided. Additionally, methods for modulating RNA targets are provided.
摘要翻译：提供了用于鉴定和/或调节涉及脆性X综合征和其它相关病症的RNA转录物和/或基因的组合物和方法。特别地，已经通过FMRP的新型单克隆抗体鉴定了脆性X智力迟钝蛋白（FMRP）的RNA靶标，并且已经鉴定了RNA结合区域的共有序列。提供了用于鉴定调节RNA靶标或相关基因的化合物，蛋白质，核苷酸等的阵列。另外，提供了调节RNA靶标的方法。

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