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1. 发明授权

US06265715B1 Non-porous membrane for MALDI-TOFMS 失效
标题翻译： MALDI-TOFMS无孔膜
公开(公告)号：US06265715B1
公开(公告)日：2001-07-24
申请号：US09239547
申请日：1999-01-29
申请人： Helene Perreault , Art Chow , Werner Ens , Harry Duckworth , Lynda Donald , Richard Oleschuk , Darren Manley , Mark McComb , Kenneth Standing , Joe O'Neil
发明人： Helene Perreault , Art Chow , Werner Ens , Harry Duckworth , Lynda Donald , Richard Oleschuk , Darren Manley , Mark McComb , Kenneth Standing , Joe O'Neil
IPC分类号： H01J4904
CPC分类号： H01J49/0418
摘要： The use of non-porous membranes as sample supports for MALDI-TOFMS analysis of peptides and proteins as well as for the analysis of whole blood is herein described. Non-porous membranes have a uniform surface, allowing for greater sensitivity and accuracy. Specifically, the non-porosity favours crystal growth on the surface of the membrane only, thereby providing enhanced spectral quality over membranes with porous structures. Studies were performed using polyurethane (PU) membranes as an example of non-porous MALDI sample supports and showed that PU membranes yielded higher quality spectra compared with porous membrane sample supports such as polyether and poly(vinyl difluoride) and the spectra obtained from PU membranes are of comparable quality as those obtained with metallic targets. However, the sample preparation for use with PU membranes is much less arduous compared with the preparation necessary for metallic targets. On membrane proteolytic digestions of proteins were performed using trypsin. After the completion of the digests, buffer components were washed of leaving peptides bound to the membrane. Addition of matrix followed by MALDI analysis resulted in good quality mass spectra which enabled peptide mapping.
摘要翻译：本文描述了使用无孔膜作为样品载体用于肽和蛋白质的MALDI-TOFMS分析以及全血的分析。无孔膜具有均匀的表面，允许更高的灵敏度和精度。具体而言，非孔隙度仅有利于膜表面的晶体生长，从而在具有多孔结构的膜上提供增强的光谱质量。使用聚氨酯（PU）膜作为非多孔MALDI样品载体的实例进行研究，并且与多孔膜样品载体如聚醚和聚（二氟乙烯）相比，PU膜产生更高质量的光谱和从PU膜获得的光谱具有与用金属靶获得的质量相当的质量。然而，与金属靶所需的制备相比，用于PU膜的样品制备要困难得多。蛋白质的膜蛋白水解消化用胰蛋白酶进行。在消化完成后，洗涤剩余肽结合到膜上的缓冲液组分。添加基质，随后进行MALDI分析，得到质谱质谱，从而进行肽图谱分析。

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