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    • 4. 发明申请
    • Method of Quickly Detecting Antigen Using Fluorescence Correlation Spectroscopy or Fluorescence Cross-Correlation Spectroscopy
    • 使用荧光相关光谱或荧光相关光谱快速检测抗原的方法
    • US20090253217A1
    • 2009-10-08
    • US12066284
    • 2006-09-08
    • Kenta SaitoHiroshi SakataFumihiko FujiiMasataka KinjoMamoru Tamura
    • Kenta SaitoHiroshi SakataFumihiko FujiiMasataka KinjoMamoru Tamura
    • G01N33/566
    • G01N33/536G01N33/582G01N33/6896G01N2021/6439G01N2800/2828
    • The present invention is to provide a method of quickly detecting an antigen at an arbitrary concentration in an antigen sample, without a multi-stage examination of the concentration ratio between a detection reagent and an antigen to be detected, particularly when the concentration of the antigen in the sample is unknown, in the method of detecting an antigen using fluorescence correlation spectroscopy (FCS) or fluorescence cross-correlation spectroscopy (FCCS). By preparing (1) a series to which only a detection reagent is added and (2) a series to which an antigen and the detection reagent are added to achieve a maximum trimer concentration, and by performing a fluorescence spectroscopic analysis, the presence or absence of the antigen in the detection sample is quickly detected by the presence or absence of a trimer detection signal from a detector in the cases of (1) and (2), in a method of detecting an antigen by FCS or FCCS using as a detection reagent a fluorescent-labeled intact antibody or fluorescent-labeled antibody fragment targeted to an epitope of the antigen to be detected, and a non-fluorescent-labeled intact antibody or fluorescent-labeled intact antibody or fluorescent-labeled antibody fragment targeted to another epitope of the antigen.
    • 本发明提供一种在抗原样品中以任意浓度快速检测抗原的方法,而不需要对检测试剂和待检测抗原之间的浓度比进行多阶段检查,特别是当抗原浓度 在样品中,在使用荧光相关光谱法(FCS)或荧光相关光谱法(FCCS)检测抗原的方法中是未知的。 通过制备(1)仅添加检测试剂的系列和(2)添加抗原和检测试剂以达到最大三聚体浓度的一系列序列,通过进行荧光光谱分析,存在或不存在 在(1)和(2)的情况下,通过检测器的三聚体检测信号的存在或不存在,通过FCS或FCCS检测抗原的方法来检测检测样品中的抗原, 试剂荧光标记的完整抗体或靶向要检测的抗原表位的荧光标记的抗体片段,以及靶向另一表位的非荧光标记的完整抗体或荧光标记的完整抗体或荧光标记的抗体片段 抗原。