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1. 发明申请

WO1997014295A1 PROCESS FOR CLONING PAPHIOPEDILA 审中-公开
标题翻译：克隆羊水过程
公开(公告)号：WO1997014295A1
公开(公告)日：1997-04-24
申请号：PCT/JP1996002998
申请日：1996-10-16
申请人： SAPPORO BREWERIES LIMITED , ZHOU, Tian, Su , TANAKA, Michio
发明人： SAPPORO BREWERIES LIMITED
IPC分类号： A01H04/00
CPC分类号： A01H4/008 , A01H4/005
摘要： A process for cloning paphiopedila by splitting surface-sterilized apical buds of the scapes or axillary shoots of a paphiopedilum or sterile shoots thereof obtained by sterile culture into fragments and culturing the same in a medium containing plant hormones. It is possible to form multishoots by splitting sterile shoots containing numerous axillary buds into fragments and culturing the same and also to effectively prevent withering caused by the browning of plant tissues by using a liquid medium containing solid supports such as rock wool or filter paper for the culture. Further it is possible to realize mass production of paphiopedila according to cloning techniques by sterilizing flower buds before flowering or by optimizing the composition of the medium and the conditions of the culture.
摘要翻译：通过将通过无菌培养获得的皮草或其无菌芽的砧木或腋芽的表面消毒的顶端芽分成片段并在含有植物激素的培养基中培养来培养罂粟的方法。可以通过将含有多个腋芽的不育芽分解成片段并进行培养来形成多重分枝，并且通过使用含有固体支持物例如岩棉或滤纸的液体培养基来有效地防止植物组织褐变引起的枯萎文化。此外，根据克隆技术，可以通过在开花前对花蕾进行消毒或通过优化培养基的组成和培养条件来实现大蒜生产。

2. 发明申请

WO1996030756A1 METHOD OF DETERMINING 'beta'-GLUCAN 审中-公开
标题翻译：测定β-GLUCAN的方法
公开(公告)号：WO1996030756A1
公开(公告)日：1996-10-03
申请号：PCT/JP1996000846
申请日：1996-03-29
申请人： SAPPORO BREWERIES LIMITED , IZAWA, Masayuki
发明人： SAPPORO BREWERIES LIMITED
IPC分类号： G01N30/06
CPC分类号： G01N30/06 , G01N30/02 , G01N35/085 , G01N2030/8435 , G01N2030/8836 , Y10T436/117497 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , B01D15/34
摘要： A method of determining beta -glucan according to the flow injection technique using calcofluor with improved precision and reproducibility, characterized by placing a gel filtration column wherein the outer volume of gel particles is smaller than the volume of eluates discharged from the column in a given period of time and the inner volume of the column is at least ten times as large as the volume of an injected sample in between the position at which the sample is injected into the liquid flow of the system and a detector.
摘要翻译：根据流式注射技术使用calcofluor测定β-葡聚糖的方法，其具有改进的精度和再现性，其特征在于放置凝胶过滤柱，其中凝胶颗粒的外部体积小于在给定时间段内从柱排出的洗脱液的体积的时间，并且柱的内部体积是在将样品注入到系统的液体流中的位置与检测器之间的注射样品的体积的至少十倍。

3. 发明申请

WO1998015612A1 METHOD OF BOILING WORT AND WORT BOILER USED THEREFOR 审中-公开
标题翻译：锅炉使用的锅炉及其锅炉的方法
公开(公告)号：WO1998015612A1
公开(公告)日：1998-04-16
申请号：PCT/JP1997003563
申请日：1997-10-06
申请人： SAPPORO BREWERIES LIMITED , MITANI, Yutaka , SUZUKI, Hidekazu , OHSHIMA, Toshiyuki , KOBAYASHI, Minoru , KURIHARA, Toshio , AKIYAMA, Hiroshi , ISHIDA, Fumito
发明人： SAPPORO BREWERIES LIMITED
IPC分类号： C12C07/22
CPC分类号： C12C7/22 , C12C9/02 , C12C13/02 , C12G1/06
摘要： A method of boiling wort which makes it possible to improve the efficiency of separation and exhalation of a wort boiler used in the preparation of beer or sparkling wine. This method is employed in the mashing step and is characterized by enhancing the extent of liquid-gas contact between wort and a gas phase in the boiler, thereby enhancing the efficiency of evaporation and exhalation of substances from the wort during the boiling and reducing the thermal energy necessary for the boiling.
摘要翻译：一种煮麦芽汁的方法，其可以提高用于制备啤酒或起泡酒的麦芽汁锅炉的分离和排出的效率。该方法用于糖化步骤，其特征在于提高了麦芽汁与锅炉中气相之间的液 - 气接触程度，从而提高了沸腾期间麦芽汁中物质的蒸发和排出效率，降低了热量沸腾所需的能量。

4. 发明申请

US20180271046A1 BARLEY LIPOXYGENASE 1 GENE, METHOD OF SELECTING BARLEY VARIETY, MATERIAL OF MALT ALCOHOLIC DRINKS AND PROCESS FOR PRODUCING MALT ALCOHOLIC DRINK 审中-公开
公开(公告)号：US20180271046A1
公开(公告)日：2018-09-27
申请号：US15979923
申请日：2018-05-15
申请人： SAPPORO BREWERIES LIMITED
发明人： Naohiko HIROTA , Takafumi KANEKO , Hisao KURODA , Hirotaka KANEDA , Kiyoshi TAKOI , Kazuyoshi TAKEDA
IPC分类号： A01H5/10 , C12Q1/6895 , C12N9/02 , C12C1/00 , A23L2/38
CPC分类号： A01H5/10 , A23L2/38 , A23V2002/00 , C12C1/00 , C12N9/0069 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： A barley plant including a mutant LOX-1 protein and that is deficient in lipoxygenase activity.

5. 发明申请

US20170295738A1 BARLEY LIPOXYGENASE 1 GENE, METHOD OF SELECTING BARLEY VARIETY, MATERIAL OF MALT ALCOHOLIC DRINKS AND PROCESS FOR PRODUCING MALT ALCOHOLIC DRINK 审中-公开
公开(公告)号：US20170295738A1
公开(公告)日：2017-10-19
申请号：US14882998
申请日：2015-10-14
申请人： SAPPORO BREWERIES LIMITED
发明人： Naohiko HIROTA , Takafumi Kaneko , Hisao Kuroda , Hirotaka Kaneda , Kiyoshi Takoi , Kazuyoshi Takeda
IPC分类号： A01H5/10 , C12Q1/68 , A23L2/38 , C12N9/02 , C12C1/00
CPC分类号： A01H5/10 , A23L2/38 , A23V2002/00 , C12C1/00 , C12N9/0069 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： A barley plant including a mutant LOX-1 protein and that is deficient in lipoxygenase activity.

6. 发明申请

US20150257354A1 BARLEY LIPOXYGENASE 1 GENE, SELECTION METHOD FOR BARLEY, MATERIALS FOR MALT ALCOHOLIC BEVERAGES AND METHOD FOR PRODUCTION OF MALT ALCOHOLIC BEVERAGES 审中-公开
标题翻译： BARLEY LIPOXYGENASE 1基因，BARLEY选择方法，用于MALT酒精饮料的材料和生产MALT酒精饮料的方法
公开(公告)号：US20150257354A1
公开(公告)日：2015-09-17
申请号：US14662416
申请日：2015-03-19
申请人： SAPPORO BREWERIES LIMITED
发明人： NAOHIKO HIROTA , TAKAFUMI KANEKO , HISAO KURODA , HIROTAKA KANEDA , KIYOSHI TAKOI , KAZUYOSHI TAKEDA
IPC分类号： A01H5/10 , A23L2/38 , C12C1/00
CPC分类号： A01H5/10 , A23L2/38 , A23V2002/00 , C12C1/00 , C12N9/0069 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： A barley plant including a mutant LOX-1 protein and that is deficient in lipoxygenase activity
摘要翻译：包括突变体LOX-1蛋白质并且缺少脂氧合酶活性的大麦植物

7. 发明申请

US20130129862A1 BARLEY LIPOXYGENASE 1 GENE, METHOD OF SELECTING BARLEY VARIETY, MATERIAL OF MALT ALCOHOLIC DRINKS AND PROCESS FOR PRODUCING MALT ALCOHOLIC DRINK 审中-公开
公开(公告)号：US20130129862A1
公开(公告)日：2013-05-23
申请号：US13667538
申请日：2012-11-02
申请人： SAPPORO BREWERIES LIMITED
发明人： Naohiko HIROTA , Takafumi Kaneko , Hisao Kuroda , Hirotaka Kaneda , Kiyoshi Takoi , Kazuyoshi Takeda
IPC分类号： A01H5/10
CPC分类号： A01H5/10 , A23L2/38 , A23V2002/00 , C12C1/00 , C12N9/0069 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： A selection method for barley lipoxygenase-1 deficient barley, comprising a step of distinguishing the barley lipoxygenase-1 deficient barley by whether or not the guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene is mutated to a different base; and a method for production of malt alcoholic beverages using a material for malt alcoholic beverages derived from barley obtained by the selection method.

8. 发明申请

US20040107763A1 Method and apparatus for monitoring oxygen concentration in a beverage production process 失效
标题翻译：用于监测饮料生产过程中的氧浓度的方法和装置
公开(公告)号：US20040107763A1
公开(公告)日：2004-06-10
申请号：US10724159
申请日：2003-12-01
申请人： SAPPORO BREWERIES LIMITED
发明人： Daisaku Kurokawa , Satoshi Sekibata , Mitsushiro Saito , Rikiya Goto
IPC分类号： G01N033/14
CPC分类号： G01N1/2226 , G01N27/404 , G01N27/407 , G01N33/14 , G01N33/146 , G01N2001/2229
摘要： The present invention continuously monitors the amount of oxygen dissolved in a beverage in a beverage storage tank in a beverage filler. Gas is continuously sampled via the vent holes 28 and the vent pipe 29 from the space part inside the beverage storage tank 1 of the beverage filler and is supplied to the oxygen measuring apparatus 40. The oxygen measuring apparatus 40 includes the oxygen measuring device 45 and measures the concentration of oxygen gas of the delivered gas. Based on the concentration of oxygen gas, it is monitored whether or not the amount of oxygen dissolved in the beer inside the storage tank is at or below a predetermined level. Further, the beverage is sampled from a supply channel supplying the beverage to the beverage storage tank so that the concentration of oxygen in the beverage is measured and monitored.
摘要翻译：本发明连续地监测饮料填充剂中饮料储罐中溶解在饮料中的氧量。气体通过通气孔28和通气管29从饮料填充剂的饮料储存罐1内部的空间部分连续取样，并供给到氧气测量装置40.氧气测量装置40包括氧气测量装置45和测量输送气体的氧气浓度。基于氧气的浓度，监测溶解在储罐内的啤酒中的氧量是否处于或低于预定水平。此外，将饮料从供应饮料的供应通道取样到饮料储存罐，以便测量和监测饮料中的氧浓度。

9. 发明申请

US20030104405A1 Method of differentiating beer yeast 失效
公开(公告)号：US20030104405A1
公开(公告)日：2003-06-05
申请号：US10067241
申请日：2002-02-07
申请人： SAPPORO BREWERIES LIMITED
发明人： Masahide Sato , Youichi Tsuchiya
IPC分类号： C07H021/04 , C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6895 , C12Q2600/158
摘要： The method of differentiating beer yeast of the invention is a method which comprises a first step of synthesizing a primer capable of amplifying the linker portion between a base sequence (A) and a base sequence (B) in a novel gene (C) which has the base sequence (B) comprising a portion of yeast chromosome IX linked downstream from the base sequence (A) comprising a portion of the N-terminal end of yeast gene Lg-FLO1, and which includes the base sequences listed as SEQ. ID. Nos. 1-6 of the Sequence Listing; a second step of carrying out a PCR (Polymerase Chain Reaction) using the primer synthesized in the first step and DNA separated from a yeast specimen; and a third step of differentiating whether the yeast is bottom-fermenting yeast or wild yeast, based on the PCR amplification product obtained from the second step.

10. 植物专利

US20200100413P1 Hop plant named 'Furano 1501U Go' 有权
公开(公告)号：US20200100413P1
公开(公告)日：2020-03-26
申请号：US16602117
申请日：2019-08-07
申请人： Sapporo Breweries Limited
发明人： Koichiro Koie , Yutaka Itoga , Mitsuhiro Uemoto , Yasunori Arai , Masanobu Goto , Seigi Kuji
IPC分类号： A01H6/00
摘要： A new hop plant particularly distinguished by having a cylindrical to club plant shape, an alpha acid content of 8.3%, a ratio content of beta to alpha of 0.58, and a citrus flavor, is disclosed.

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