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1. 发明申请

WO2009093200A3 PRODUCTION OF PROGENITOR CEREAL CELLS 审中-公开
标题翻译：生产谷胱甘肽原
公开(公告)号：WO2009093200A3
公开(公告)日：2009-11-05
申请号：PCT/IB2009050260
申请日：2009-01-23
申请人： CSIR , PIONEER HI BRED INT , MEHLO LUKE , ZUO-YU ZHAO
发明人： MEHLO LUKE , ZUO-YU ZHAO
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , A01H4/005 , A01H4/008
摘要： A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4- dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.
摘要翻译：描述了用于从未分化愈伤组织细胞产生和维持多能和/或全能祖先谷物细胞的方法。在含有至少一种生长素和至少一种细胞分裂素的培养基上通过直接器官发生进行祖细胞的产生。例如，生长素可以是2,4-二氯苯氧基乙酸，吲哚-3-乙酸，吡咯兰，萘平乙酸，吲哚-3-丙酸，吲哚-3-丁酸，苯乙酸，苯并呋喃-3-乙酸或苯基丁酸，细胞分裂素可以是苄基氨基嘌呤，苄基腺嘌呤，噻替隆，玉米素，异戊二醛，反式玉米素或二甲基烯丙基。还描述了用于转化未分化愈伤组织细胞和/或祖细胞谷粒细胞的方法。典型的谷类细胞是高粱，玉米，小麦，大麦，小米，黑麦，卡诺拉，苜蓿，黑小麦和水稻。

2. 发明申请

WO2009093201A3 METHOD FOR REGULATING AGROBACTERIUM-MEDIATED TRANSFORMATION 审中-公开
标题翻译：调节农杆菌介导转化的方法
公开(公告)号：WO2009093201A3
公开(公告)日：2009-09-17
申请号：PCT/IB2009050261
申请日：2009-01-23
申请人： CSIR , PIONEER HI BRED INT , MEHLO LUKE , ZUO-YU ZHAO
发明人： MEHLO LUKE , ZUO-YU ZHAO
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
摘要翻译：描述了调节农杆菌毒力基因表达的方法。该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。

3. 发明申请

WO2009093201A2 METHOD FOR REGULATING AGROBACTERIUM-MEDIATED TRANSFORMATION 审中-公开
标题翻译：调节农杆菌介导的转化的方法
公开(公告)号：WO2009093201A2
公开(公告)日：2009-07-30
申请号：PCT/IB2009/050261
申请日：2009-01-23
申请人： CSIR , PIONEER HI-BRED INTERNATIONAL, INC. , MEHLO, Luke , ZUO-YU, Zhao
发明人： MEHLO, Luke , ZUO-YU, Zhao
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium , effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
摘要翻译：描述了调节农杆菌毒力基因表达的方法。该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚类的化合物，并使土壤杆菌接触该化合物。该化合物诱导土壤杆菌毒力基因的表达，从土壤杆菌转移到谷物细胞，由此转化T-DNA。

4. 发明申请

WO2009093200A9 PRODUCTION OF PROGENITOR CEREAL CELLS 审中-公开
标题翻译：生产祖先细胞
公开(公告)号：WO2009093200A9
公开(公告)日：2009-12-23
申请号：PCT/IB2009050260
申请日：2009-01-23
申请人： CSIR , PIONEER HI BRED INT , MEHLO LUKE , ZUO-YU ZHAO
发明人： MEHLO LUKE , ZUO-YU ZHAO
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , A01H4/005 , A01H4/008
摘要： A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4- dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.
摘要翻译：描述了用于从未分化的愈伤组织细胞生产和维持多能和/或全能祖细胞细胞的方法。通过在含有至少一种生长素和至少一种细胞分裂素的培养基上的直接器官发生来产生祖细胞。例如，生长素可以是2,4-二氯苯氧乙酸，吲哚-3-乙酸，picloran，萘乙酸，吲哚-3-丙酸，吲哚-3-丁酸，苯乙酸，苯并呋喃-3-乙酸或苯基丁酸，细胞分裂素可以是苄基氨基嘌呤，苄基腺嘌呤，噻苯隆，玉米素，异戊基腺嘌呤，反式玉米素或二甲基烯丙基腺嘌呤。还描述了未分化愈伤组织细胞和/或祖先谷物细胞的转化过程。典型的谷类细胞是高粱，玉米，小麦，大麦，小米，黑麦，卡诺拉油菜，苜蓿，黑小麦和稻米。

5. 发明申请

WO2009093200A2 PRODUCTION OF PROGENITOR CEREAL CELLS 审中-公开
标题翻译：生产祖先细胞
公开(公告)号：WO2009093200A2
公开(公告)日：2009-07-30
申请号：PCT/IB2009/050260
申请日：2009-01-23
申请人： CSIR , PIONEER HI-BRED INTERNATIONAL, INC. , MEHLO, Luke , ZUO-YU, Zhao
发明人： MEHLO, Luke , ZUO-YU, Zhao
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , A01H4/005 , A01H4/008
摘要： A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4- dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.
摘要翻译：描述了用于从未分化愈伤组织细胞生产和维持多能和/或全能祖细胞细胞的方法。通过在含有至少一种生长素和至少一种细胞分裂素的培养基上的直接器官发生来产生祖细胞。例如，生长素可以是2,4-二氯苯氧乙酸，吲哚-3-乙酸，picloran，萘乙酸，吲哚-3-丙酸，吲哚-3-丁酸，苯乙酸，苯并呋喃-3-乙酸或苯基丁酸，细胞分裂素可以是苄基氨基嘌呤，苄基腺嘌呤，噻苯隆，玉米素，异戊基腺嘌呤，反式玉米素或二甲基烯丙基腺嘌呤。还描述了未分化愈伤组织细胞和/或祖先谷物细胞的转化过程。典型的谷类细胞是高粱，玉米，小麦，大麦，小米，黑麦，卡诺拉油菜，苜蓿，黑小麦和大米。

6. 发明授权

US08143484B2 Method for regulating agrobacterium-mediated transformation 有权
标题翻译：调节农杆菌介导转化的方法
公开(公告)号：US08143484B2
公开(公告)日：2012-03-27
申请号：US12357284
申请日：2009-01-21
申请人： Luke Mehlo , Zuo-Yu Zhao
发明人： Luke Mehlo , Zuo-Yu Zhao
IPC分类号： C12N15/84
CPC分类号： C12N15/8205
摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
摘要翻译：描述了调节农杆菌毒力基因表达的方法。该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。

7. 发明申请

US20100192253A1 METHODS FOR IMPROVING MONOCOT TRANSFORMATION 失效
标题翻译：改进单转换方法
公开(公告)号：US20100192253A1
公开(公告)日：2010-07-29
申请号：US12694013
申请日：2010-01-26
申请人： XINLI E. WU , MYEONG-JE CHO , ZUO-YU ZHAO
发明人： XINLI E. WU , MYEONG-JE CHO , ZUO-YU ZHAO
IPC分类号： C12N15/82 , C12N5/02 , A01H1/00
CPC分类号： C12N15/8205
摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.
摘要翻译：本发明提供改进的植物转化方法。特别地，该方法提供增加的转化频率，特别是在顽固植物中。该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。

8. 发明授权

US06720475B1 Nucleic acid sequence encoding FLP recombinase 有权
标题翻译：编码FLP重组酶的核酸序列
公开(公告)号：US06720475B1
公开(公告)日：2004-04-13
申请号：US09641111
申请日：2000-08-17
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号： A01H500
CPC分类号： C12N15/8289 , C12N9/00 , C12N15/8213
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译：在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。

9. 发明授权

US08030556B2 Highly transformable elite doubled haploid line PH17AW 有权
标题翻译：高度可转化的精英双倍体单倍体PH17AW
公开(公告)号：US08030556B2
公开(公告)日：2011-10-04
申请号：US12174812
申请日：2008-07-17
申请人： Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
发明人： Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
IPC分类号： A01H5/00 , A01H5/10 , A01H1/02 , C12N5/04
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PH17AW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PH17AW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH17AW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PH17AW or a trait conversion of PH17AW with another maize line. Inbred maize lines derived from double haploid maize line PH17AW, methods for producing other inbred maize lines derived from double haploid maize line PH17AW and the inbred maize lines and their parts derived by those methods.
摘要翻译：一种新型双倍体玉米品系，命名为PH17AW，其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍单倍体玉米品系PH17AW。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗入PH17AW的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。杂交玉米种子，植物或植物部分通过双重单倍体系PH17AW或PH17AW与另一个玉米品系的转化而产生。衍生自双倍体单倍体玉米品系PH17AW的近交玉米品系，从双倍体单倍体玉米品系PH17AW生产其他近交玉米品系的方法和由这些方法衍生的近交玉米品系及其部分。

10. 发明授权

US07999158B2 Highly transformable elite inbred line-PHWWE 有权
标题翻译：高度可转型的精英近交系 - PHWWE
公开(公告)号：US07999158B2
公开(公告)日：2011-08-16
申请号：US12534507
申请日：2009-08-03
申请人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
发明人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
IPC分类号： A01H5/00 , A01H5/10 , A01H1/02 , C12N5/04
CPC分类号： A01H5/10
摘要： A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
摘要翻译：一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此生产的玉米种子，植物和植物部分。杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。

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