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    • 10. 发明专利
    • Dna array binding cell surface marker genes of human and rat, and application thereof
    • 人类和大鼠的DNA阵列结合细胞表面标记基因及其应用
    • JP2009050182A
    • 2009-03-12
    • JP2007218030
    • 2007-08-24
    • Okayama Univ国立大学法人 岡山大学
    • SENOO SHOJI
    • C12N15/09C12M1/00C12Q1/68
    • PROBLEM TO BE SOLVED: To rapidly and comprehensively detect the expression of a cell surface protein genetic cluster of mammals.
      SOLUTION: This method for designing a probe for the cell surface protein genetic cluster of the mammals comprises designing the probe according to following standards: (1) a step of selecting a transmembrane region or a GPI anchor modification site of the cell surface protein, (2) a step of determining the probe of 50-70 mers when the base sequence of 50-70 mers containing the selected transmembrane region or GPI anchor modification site has 40-60% GC content, (3) a step of designing the probe having the 40-60% GC content according to the order of a specific process when the cell surface protein has the transmembrane region and the base sequence of 50-70 mers containing the transmembrane region can not have the 40-60% GC content and (4) a step of designing the probe having the 40-60% GC content according to the order of the specific process when the cell surface protein has the GPI anchor modification site and the base sequence of 50-70 mers containing the GPI anchor modification site can not have the 40-60% GC content.
      COPYRIGHT: (C)2009,JPO&INPIT
    • 要解决的问题:快速全面地检测哺乳动物细胞表面蛋白遗传簇的表达。 解决方案:用于设计哺乳动物细胞表面蛋白质遗传簇的探针的方法包括根据以下标准设计探针:(1)选择细胞表面的跨膜区或GPI锚修饰位点的步骤 蛋白质,(2)当含有选择的跨膜区或GPI锚修饰位点的50-70mM的碱基序列具有40-60%的GC含量时,确定50-70mM的探针的步骤,(3)设计步骤 当细胞表面蛋白具有跨膜区并且含有跨膜区的50-70mer的碱基序列不具有40-60%的GC含量时,探针具有根据具体过程的顺序的40-60%GC含量 和(4)当细胞表面蛋白具有GPI锚修饰位点和含有GPI锚的50-70个碱基序列时,根据具体过程的顺序设计具有40-60%GC含量的探针的步骤 修改si te不能有40-60%的GC含量。 版权所有(C)2009,JPO&INPIT