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    • 3. 发明公开
    • 벼 유래의 OsZIP1 유전자 및 단백질
    • 从ORYZA SATIVA衍生的OSZIP1基因和蛋白质
    • KR1020100130724A
    • 2010-12-14
    • KR1020090049390
    • 2009-06-04
    • 대한민국(농촌진흥청장)
    • 박상렬변명옥문석준한세연박수철신동진조우석이성곤김범기윤혜진정미정권택륜
    • C07K14/425C12N15/29A01H1/00
    • PURPOSE: A rice plant-derived OsZIP1 protein and nucleic acid molecule encoding the same are provided to ensure disease resistance and to induce phenotype of deep flower color. CONSTITUTION: An OsZIP1 protein has an amino acid sequence of sequence number 1. The OsZIP1 protein relates to plant disease resistance, growth suppression or flower color enhancement. A nucleic acid molecule encoding the OsZIP1 protein has a nucleotide sequence of sequence number 1. A method for producing a transgenic plant having the plant disease resistance, growth suppression or flower color enhancement comprises: a step of transforming a plant cells or tissue with a recombinant vector having OsZIP1 gene; a step of selecting transformed plant cells or tissue; and a step of redifferentiating the plant from the plant cells or tissue.
    • 目的:提供水稻植物衍生的OsZIP1蛋白和编码它的核酸分子,以确保抗病性并诱导深花色的表型。 构成:OsZIP1蛋白具有序列号1的氨基酸序列。OsZIP1蛋白涉及植物病害抗性,生长抑制或花色增强。 编码OsZIP1蛋白的核酸分子具有序列号1的核苷酸序列。具有植物抗病性,生长抑制或花色增强的转基因植物的制备方法包括:用重组体转化植物细胞或组织的步骤 载体具有OsZIP1基因; 选择转化的植物细胞或组织的步骤; 以及将植物从植物细胞或组织重新分化的步骤。
    • 6. 发明公开
    • 감자 한발 적응 유전자 및 이 유전자로 형질전환 된 내한발성 감자
    • 使用相同的磷酸盐和耐腐植物脱水耐性相关基因
    • KR1020040050634A
    • 2004-06-16
    • KR1020020078495
    • 2002-12-10
    • 대한민국(농촌진흥청장)
    • 변명옥송혜숙배신철박재복김둘이이기완이승범정미정이부영권혁빈
    • C12N15/29
    • C12N15/8273
    • PURPOSE: A gene related to dehydration tolerance of potato and dehydration-tolerant plant using the same are provided, thereby amplifying the expression of the gene when the plant is faced with dehydration, so that the plant can grow under dehydration condition. CONSTITUTION: The gene StRD22 related to dehydration tolerance of potato is isolated from potato and has the nucleotide sequence set forth in SEQ ID NO: 1, wherein the gene StRD22 is isolated by the steps of: freezing potato treated at 4 deg. C for 8 hours and untreated potato and pulverizing them; extracting RNA from the pulverized potatoes; isolating poly A+RNA from the extracted total RNA; subjecting the poly A+RNA to RNase H-reverse transcriptase to synthesize first strand cDNA; removing RNA using RNase and synthesizing second strand cDNA using DNA polymerase I; inserting the synthesized cDNA into Uni-zap XR vector; in vitro packaging the cDNA inserted vector; infecting E. coli with the cDNA inserted vector to determine Phage titer and amplify the genes; and sequencing the genes.
    • 目的:提供一种与马铃薯和脱水耐受性相关的脱水耐性相关基因,从而在植物面临脱水时扩增基因的表达,使植物在脱水条件下生长。 构成:与马铃薯的脱水耐性相关的基因StRD22与马铃薯分离,具有SEQ ID NO:1所示的核苷酸序列,其中通过以下步骤分离基因StRD22:将4℃处理的马铃薯冷冻。 8小时,未处理的马铃薯并粉碎; 从粉碎的马铃薯中提取RNA; 从提取的总RNA中分离聚A + RNA; 将聚A + RNA转染至RNA酶H逆转录酶合成第一链cDNA; 使用RNA酶除去RNA并使用DNA聚合酶I合成第二链cDNA; 将合成的cDNA插入到Uni-zap XR载体中; 体外包装cDNA插入载体; 用cDNA插入载体感染大肠杆菌以确定噬菌体滴度并扩增基因; 并对基因进行测序。
    • 9. 发明公开
    • 느타리 버섯으로부터 분리된 신규한 GPD유전자를 형질전환시킨 오스모틱 스트레스 내성 효모
    • 由包含从PLEUROTUS SAJOR-CAJU分离的GPD基因的重组载体转化的耐摩擦重组酵母
    • KR1020010011673A
    • 2001-02-15
    • KR1019990031152
    • 1999-07-29
    • 대한민국(농촌진흥청장)
    • 정미정박수철권혁빈변명옥류진창
    • C12N15/81
    • C12N15/81C12N1/16C12R1/85
    • PURPOSE: An osmotic stress resistant yeast transformed by a recombinant vector containing the GPD gene that isolated from Pleurotus sajor-caju is provided for improving the osmotic stress resistance of yeast. CONSTITUTION: The osmotic stress resistant yeast transformed by a recombinant vector containing glycerol-3-phosphate dehydrogenase(GPD) gene as an osmotic stress related gene is obtained by the following steps of: incubating Pleurotus sajor-caju ASI 2070 in an MCM medium containing 20g of peptone, 2g of yeast extract, 2g of K2HPO4, 1.0g of KH2PO4, 0.46g of MgSO47H2O, 0.5g of DW1L at 25 deg. C for 15 days; filtering with mira cloth; separating total RNA and DNA of Pleurotus sajor-caju ASI 2070 by using a Graham method and a Sambrook method, respectively; preparing the pYES2 vector containing the GPD gene by using restriction enzymes; and transforming Saccharomyces cerevisiae INVSC2 with the pYES2 vector.
    • 目的:提供通过含有从侧耳属sajor-caju分离的GPD基因的重组载体转化的渗透胁迫酵母,用于改善酵母的渗透胁迫性。 构成:通过含有甘油-3-磷酸脱氢酶(GPD)基因的重组载体转化的渗透压胁迫酵母作为渗透胁迫相关基因,通过以下步骤获得:将Pleurotus sajor-caju ASI 2070在含有20g的MCM培养基中培养 的蛋白胨,2g酵母提取物,2g K 2 HPO 4,1.0g KH 2 PO 4,0.46g MgSO 4·7H 2 O,0.5g DW1L,25℃。 C 15天; 用米拉布过滤; 分别使用Graham方法和Sambrook方法分离了侧耳菇sajor-caju ASI 2070的总RNA和DNA; 通过使用限制酶制备含有GPD基因的pYES2载体; 并用pYES2载体转化酿酒酵母INVSC2。