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    • 6. 发明申请
    • Method for screening for bacterial conjunctivitis
    • 筛选细菌性结膜炎的方法
    • US20070140971A1
    • 2007-06-21
    • US11303001
    • 2005-12-15
    • John MacDonaldStephanie MartinJason Lye
    • John MacDonaldStephanie MartinJason Lye
    • A61K49/00C12Q1/04
    • C12Q1/04C12Q1/14
    • A method for rapidly detecting infectious conjunctivitis in a host is provided. The method includes contacting an ocular test sample with a chromogen (e.g., Reichardt's dye) that exhibits a color change in the presence of a microbe. The present inventors have discovered that the extent of the color change may vary depending on whether the microbe is a bacteria or virus. Without intending to be limited by theory, the present inventors believe that the chromogen interacts with the peptidoglycan-based cell wall structure of bacteria to induce a color change that is even more apparent at infectious levels. It is believed that this interaction occurs to a much greater extent in bacteria than in viruses. Accordingly, although the chromogen may still undergo a color change in the presence of the viruses, it is typically to a much lesser extent. In this manner, the degree of color change of the chromogen may be used in the present invention as a mechanism for differentiating between viral and bacterial conjunctivitis.
    • 提供了一种快速检测宿主感染性结膜炎的方法。 该方法包括使眼睛测试样品与在微生物存在下显示颜色变化的色原体(例如,Reichardt's染料)接触。 本发明人已经发现,颜色变化的程度可以根据微生物是细菌还是病毒而变化。 不期望受理论的限制,本发明人相信色原体与细菌的基于聚肽聚糖的细胞壁结构相互作用以诱导在感染水平上更加明显的颜色变化。 相信这种相互作用在细菌中比在病毒中更大程度地发生。 因此,虽然染色体在病毒存在的情况下仍可能发生颜色变化,但通常其程度要小得多。 以这种方式,在本发明中可以使用色原体的颜色变化程度作为区分病毒和细菌性结膜炎的机制。
    • 8. 发明申请
    • Technique for detecting microorganisms
    • 微生物检测技术
    • US20060223052A1
    • 2006-10-05
    • US11094807
    • 2005-03-30
    • John MacDonaldRichard Borders
    • John MacDonaldRichard Borders
    • C12Q1/70C12Q1/04
    • C12Q1/04G01N33/523
    • A technique for detecting the presence of microorganisms in a simple, rapid, and efficient manner is provided. More specifically, the technique involves identifying one or more volatile compounds associated with a particular microorganism of interest. The volatile compounds may be identified, for instance, using solid phase microextraction in conjunction with gas chromatography/mass spectroscopy (“GC/MS”) analysis methods. Once identified, an indicator may then be selected that is configured to undergo a detectable color change in the presence of the identified volatile compound(s). If desired, the indicator may be provided on a substrate to form an indicator strip for use in a wide variety of applications. In this manner, the presence of the microorganism may be rapidly detected by simply observing a color change of the indicator strip.
    • 提供了以简单,快速和有效的方式检测微生物的存在的技术。 更具体地,该技术涉及鉴定与感兴趣的特定微生物相关联的一种或多种挥发性化合物。 可以鉴定挥发性化合物,例如使用固相微萃取与气相色谱/质谱联用(“GC / MS”)分析方法。 一旦鉴定,可以选择配置为在所鉴定的挥发性化合物存在下进行可检测的颜色变化的指示剂。 如果需要,指示器可以设置在基板上以形成用于各种应用中的指示条。 以这种方式,可以通过简单地观察指示条的颜色变化来快速检测微生物的存在。