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    • 10. 发明授权
    • Recombinant microorganisms having modified production of alcohols and acids
    • 具有醇和酸改性产生的重组微生物
    • US08039239B2
    • 2011-10-18
    • US12336278
    • 2008-12-16
    • Andrew Reeves
    • Andrew Reeves
    • C12P7/06C12P7/16C12N9/04C12N1/20C01B3/00
    • C12P7/065C12P7/04C12P7/16Y02E50/10Y02E50/17
    • Recombinant acetogenic Clostridia are engineered to modulate production of aliphatic C2-C6 alcohols and aliphatic C2-C6 organic acids from synthetic gases. One aspect of the invention provides a method of producing an aliphatic C2-C6 alcohol using an acetogenic Clostridium micro-organism having at least one genetic modification that reduces or eliminates C2-C6 carboxylic acid production by the modified organism. Another aspect of the invention provides a method of producing an aliphatic C2-C6 alcohol using an acetogenic Clostridium micro-organism having one or more genetic modifications that cause increased enzyme activity of carbon monoxide dehydrogenase, aldehyde ferredoxin oxidoreductase, NADPH-dependent alcohol dehydrogenase, or alcohol dehydrogenase. Yet another aspect of the invention provides a method of producing aliphatic C2-C6 alcohols using acetogenic Clostridium micro-organisms that have been genetically modified to increase C2-C6 aliphatic alcohol formation and decrease the production of the corresponding C2-C6 aliphatic organic acid.
    • 工程改造重组乙型梭菌用于调控来自合成气体的脂族C 2 -C 6醇和脂族C 2 -C 6有机酸的生产。 本发明的一个方面提供了使用具有减少或消除经修饰的生物体的C2-C6羧酸产生的至少一种遗传修饰的产乙酰梭菌微生物来生产脂族C 2 -C 6醇的方法。 本发明的另一方面提供了一种使用具有一种或多种遗传修饰的乙酰酵母梭菌微生物来生产脂族C 2 -C 6醇的方法,所述遗传修饰引起一氧化碳脱氢酶,醛铁氧还蛋白氧化还原酶,NADPH依赖性醇脱氢酶的酶活性增加,或 醇脱氢酶。 本发明的另一方面提供了一种使用已经被遗传修饰以产生C2-C6脂族醇形成并降低对应的C 2 -C 6脂族有机酸的产生的乙酰酵母梭菌微生物来生产脂族C 2 -C 6醇的方法。