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    • 4. 发明授权
    • Process for purification of proanthocyanidin oligomer
    • 纯化原花青素低聚物的方法
    • US06800433B1
    • 2004-10-05
    • US09959274
    • 2001-10-19
    • Shinkichi HondaTomoya TakahashiAyako KamimuraTakako MatsuokaTomomasa KandaAkio YanagidaKazuo Hieda
    • Shinkichi HondaTomoya TakahashiAyako KamimuraTakako MatsuokaTomomasa KandaAkio YanagidaKazuo Hieda
    • C12Q100
    • C07D311/62C07D311/60
    • The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products there from by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.
    • 本发明涉及一种纯化二聚至五聚原花青素低聚物的方法,该方法包括通过使用乙酸甲酯作为液相通过固液萃取从原料中提取原花青素低聚物或粗提纯产物; 用于纯化二聚体至五聚原花青素低聚物的方法,其包括用酶预处理含有原花青素低聚物的水解原料,其粗提纯产物或含有这些之一的溶液; 以及具有均匀聚合度的二聚至五聚原花青素低聚物的纯化方法,该方法包括通过使用作为流动相的正相硅胶液相色谱法从含有原花青素低聚物或粗纯化产物的原料中分离纯化原花青素低聚物 将溶剂选自酯溶剂,酮溶剂,烃溶剂,醚溶剂和醇溶剂中的两种或更多种溶剂的单一溶剂或混合溶剂。
    • 5. 发明申请
    • Method for Preparation of Plant Extract
    • 植物提取物的制备方法
    • US20090053384A1
    • 2009-02-26
    • US11887777
    • 2006-03-16
    • Takehiro WatanabeRumi FujitaMotoyuki TagashiraTomomasa KandaSeiichi HiguchiMasayuki Tanabe
    • Takehiro WatanabeRumi FujitaMotoyuki TagashiraTomomasa KandaSeiichi HiguchiMasayuki Tanabe
    • C12C3/00A23L1/28A23L1/025
    • C07G17/00A23L33/105C12F3/06
    • It is intended to develop a technique for producing a water-soluble polyphenol at high purity and with high efficiency in a short time from hop bract or the like which is a by-product in the beer brewing. A method for preparation of a purified plant extract comprising steps of treating a plant extract with a clay mineral such as bentonite and removing any divalent cations from the resulting product; a method for producing a polyphenol comprising steps of passing the purified plant extract through a column to adsorb polyphenols onto the column and passing a solvent though the column to elute a water-soluble polyphenol from the column; and a method for producing a water-soluble hop bract polyphenol comprising steps of adding bentonite to a hop bract extract and allowing the resulting mixture to stand under acidic conditions, performing solid-liquid separation to produce a supernatant, purifying the supernatant through a column to give a pholyphenol-containing fraction, cooling the fraction and allowing the cooled fraction to stand to cause the precipitation of water-insoluble ingredients and performing solid-liquid separation to give the desired water-soluble hop bract polyphenol.
    • 旨在开发一种在啤酒酿造中作为副产物的啤酒花苞等在高纯度和高效率的短时间内生产水溶性多酚的技术。 一种纯化植物提取物的制备方法,其包括用粘土矿物如膨润土处理植物提取物并从所得产物中除去任何二价阳离子的步骤; 一种生产多酚的方法包括以下步骤:使纯化的植物提取物通过柱,以将多酚吸附到柱上并使溶剂通过柱,以从柱中洗脱水溶性多酚; 以及制造水溶性跳苞片多酚的方法,其特征在于,包括以下步骤:将膨润土加入到鞭毛提取物中,使所得混合物在酸性条件下放置,进行固液分离,制成上清液,通过柱纯化上清液至 得到含苯酚的级分,冷却该级分并使冷却的级分静置以引起水不溶性成分的沉淀并进行固液分离,得到所需的水溶性鞭毛多酚。