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1. 发明授权

US07132243B2 Clonal expansion of T cells of unknown specificity and identification of ligand recognized by the clonally expanded T cells 有权
标题翻译：未知特异性T细胞的克隆扩增和克隆扩增的T细胞识别的配体的鉴定
公开(公告)号：US07132243B2
公开(公告)日：2006-11-07
申请号：US09962445
申请日：2001-09-26
申请人： Sho Matsushita , Toshihiro Nakashima
发明人： Sho Matsushita , Toshihiro Nakashima
IPC分类号： G01N33/53 , G01N33/566 , C12N5/08
CPC分类号： C12N5/0636 , C12N2501/23 , C12N2501/58 , C12N2503/00 , G01N33/505
摘要： A process for clonally expanding T cells of unknown specificity and a process for identifying a peptide ligand recognized by said T cells are provided.A process for clonally expanding T cells of unknown specificity which comprises co-culturing said T cells of unknown specificity with combinatorial randomized peptide library (Xn peptide library wherein n is a number of amino acids) consisted of peptides having a randomized amino acid sequence consisted of amino acid residues selected from nineteen kinds of naturally occurring amino acid other than cysteine, an interleukin, and major histocompatibility complex (MHC) class II antigen-expressing cells with DNA synthesis being suspended, said cells being derived from the individual where said T cells are obtained; and a process for identifying a peptide ligand recognized by the T cell clone of unknown specificity obtained by said process, which comprises determining proliferating activity of said T cell clone with Xn peptide library, in the presence of an interleukin and MHC class II antigen-expressing cells with DNA synthesis being suspended, said cells being derived from the individual where said T cells are obtained, to thereby determine a peptide sequence that can activate proliferation of T cells.
摘要翻译：提供了用于克隆扩增未知特异性的T细胞的方法和用于鉴定所述T细胞识别的肽配体的方法。一种用于克隆扩增未知特异性的T细胞的方法，其包括由具有随机化氨基酸序列的肽组成的组合随机肽文库（Xn肽文库，其中n为多个氨基酸）共同培养所述具有未知特异性的T细胞选自十九种除了半胱氨酸之外的天然存在的氨基酸，白细胞介素和悬浮有DNA合成的主要组织相容性复合体（MHC）II类抗原表达细胞的氨基酸残基，所述细胞衍生自所述T细胞的个体获得; 以及用于鉴定由所述方法获得的未知特异性的T细胞克隆识别的肽配体的方法，其包括在存在白介素和MHC II类抗原表达的情况下，用Xn肽文库测定所述T细胞克隆的增殖活性悬浮有DNA合成的细胞，所述细胞衍生自获得所述T细胞的个体，从而确定可以激活T细胞增殖的肽序列。

2. 发明授权

US08993254B2 Methods for evaluating risk of mother's milk or food inducing an onset of atopic dermatitis, and mother's milk or food with reduced risk of inducing an onset of atopic dermatitis 有权
标题翻译：用于评估母乳或诱发特应性皮炎发作的食物的风险的方法，以及降低诱发特应性皮炎发作风险的母乳或食物
公开(公告)号：US08993254B2
公开(公告)日：2015-03-31
申请号：US13504413
申请日：2010-10-26
申请人： Sho Matsushita , Takehiro Higashi
发明人： Sho Matsushita , Takehiro Higashi
IPC分类号： G01N33/00 , G01N33/68
CPC分类号： G01N33/6893 , G01N2333/90 , G01N2800/02 , G01N2800/202 , Y10S436/824
摘要： An entity of a Th2 adjuvant activity in mother's milk has been revealed as coenzyme A by HPLC and mass spectrometry. The followings have been found out that: a risk of developing atopic dermatitis can be evaluated by targeting coenzyme A; and any one of a food and mother's milk with a reduced risk of developing atopic dermatitis can be prepared by removing or inactivating coenzyme A.
摘要翻译：母乳中Th2佐剂活性的一个实体已经通过HPLC和质谱显示为辅酶A。以下已经发现：可以通过靶向辅酶A来评估发生特应性皮炎的风险; 可以通过去除或灭活辅酶A来制备具有降低发生特应性皮炎风险的食物和母乳中的任何一种。

3. 发明申请

US20120214183A1 METHODS FOR EVALUATING RISK OF MOTHER'S MILK OR FOOD INDUCING AN ONSET OF ATOPIC DERMATITIS, AND MOTHER'S MILK OR FOOD WITH REDUCED RISK OF INDUCING AN ONSET OF ATOPIC DERMATITIS 有权
标题翻译：用于评估母乳或食物诱发原发性肥胖的风险的方法，以及母亲的奶或食物，降低诱发原发性乳腺疾病的风险
公开(公告)号：US20120214183A1
公开(公告)日：2012-08-23
申请号：US13504413
申请日：2010-10-26
申请人： Sho Matsushita , Takehiro Higashi
发明人： Sho Matsushita , Takehiro Higashi
IPC分类号： G01N33/53 , G01N30/00 , C12M1/34 , G01N33/566
CPC分类号： G01N33/6893 , G01N2333/90 , G01N2800/02 , G01N2800/202 , Y10S436/824
摘要： An entity of a Th2 adjuvant activity in mother's milk has been revealed as coenzyme A by HPLC and mass spectrometry. The followings have been found out that: a risk of developing atopic dermatitis can be evaluated by targeting coenzyme A; and any one of a food and mother's milk with a reduced risk of developing atopic dermatitis can be prepared by removing or inactivating coenzyme A.
摘要翻译：母乳中Th2佐剂活性的一个实体已经通过HPLC和质谱显示为辅酶A。以下已经发现：可以通过靶向辅酶A来评估发生特应性皮炎的风险; 可以通过去除或灭活辅酶A来制备具有降低发生特应性皮炎风险的食物和母乳中的任何一种。

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