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1. 发明申请

US20060292578A1 DNA polymerase blends and mutant DNA polymerases 审中-公开
标题翻译： DNA聚合酶共混物和突变型DNA聚合酶
公开(公告)号：US20060292578A1
公开(公告)日：2006-12-28
申请号：US11170762
申请日：2005-06-28
申请人： Weidong Zheng , Jun Lee , Robert Potter , David Mandelman
发明人： Weidong Zheng , Jun Lee , Robert Potter , David Mandelman
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12P19/34 , C12N9/22 , C12N15/74 , C12N1/21
CPC分类号： C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6806 , C12Q1/686 , C12Y207/07007 , Y02P20/52 , C12Q2561/101 , C12Q2527/149 , C12Q2521/101
摘要： A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,
摘要翻译：一种热稳定DNA聚合酶组合物，其包含至少两种DNA聚合酶，其中之一基本上降低了5'-核酸外切酶活性，其中一种具有5'-核酸外切酶活性。该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法，

2. 发明申请

US20070059713A1 SSB-DNA polymerase fusion proteins 审中-公开
标题翻译： SSB-DNA聚合酶融合蛋白
公开(公告)号：US20070059713A1
公开(公告)日：2007-03-15
申请号：US11222029
申请日：2005-09-09
申请人： Jun Lee , Robert Potter , David Mandelman
发明人： Jun Lee , Robert Potter , David Mandelman
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12N9/22 , C12N15/74
CPC分类号： C12Q1/6806 , C12N9/1252 , C12N9/1276 , C12N15/62 , C12Q1/6848 , C12Q2522/101
摘要： Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
摘要翻译：包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。

3. 发明授权

US08828700B2 SSB-polymerase fusion proteins 有权
标题翻译： SSB聚合酶融合蛋白
公开(公告)号：US08828700B2
公开(公告)日：2014-09-09
申请号：US12326048
申请日：2008-12-01
申请人： Jun Lee , Robert Potter , David Mandelman
发明人： Jun Lee , Robert Potter , David Mandelman
IPC分类号： C12N9/12 , C12Q1/68 , C12N15/62
CPC分类号： C12Q1/6806 , C12N9/1252 , C12N9/1276 , C12N15/62 , C12Q1/6848 , C12Q2522/101
摘要： Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
摘要翻译：包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。

4. 发明授权

US08753845B2 Thermostable reverse transcriptases and uses thereof 有权
标题翻译：热稳定逆转录酶及其用途
公开(公告)号：US08753845B2
公开(公告)日：2014-06-17
申请号：US12979899
申请日：2010-12-28
申请人： Gulshan Dhariwal , Gary Gerard , Jun Lee , Robert Potter , Kim Rosenthal , Michael D. Smith
发明人： Gulshan Dhariwal , Gary Gerard , Jun Lee , Robert Potter , Kim Rosenthal , Michael D. Smith
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12N9/1276 , C12P19/34 , C12Y207/07049
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

5. 发明申请

US20060275792A1 Enhancement of nucleic acid amplification using double-stranded DNA binding proteins 审中-公开
标题翻译：使用双链DNA结合蛋白增强核酸扩增
公开(公告)号：US20060275792A1
公开(公告)日：2006-12-07
申请号：US11365383
申请日：2006-02-28
申请人： Jun Lee , Robert Potter , Sandrine Javorschi-Miller
发明人： Jun Lee , Robert Potter , Sandrine Javorschi-Miller
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6848 , C12Q2563/107 , C12Q2535/125 , C12Q2525/15
摘要： Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.
摘要翻译：公开了用于增强核酸扩增反应的效率，特异性和/或特异性的方法和材料（例如，PCR扩增反应）。所公开的发明涉及在扩增反应中使用非特异性双链DNA结合蛋白。本文描述的方法可用于各种分子生物学应用，包括等位基因特异性PCR。

6. 发明申请

US20070141592A1 Thermostable reverse transcriptases and uses thereof 审中-公开
标题翻译：热稳定逆转录酶及其用途
公开(公告)号：US20070141592A1
公开(公告)日：2007-06-21
申请号：US11492194
申请日：2006-07-25
申请人： Michael Smith , Robert Potter , Gulshan Dhariwal , Gary Gerard , Kim Rosenthal , Jun Lee
发明人： Michael Smith , Robert Potter , Gulshan Dhariwal , Gary Gerard , Kim Rosenthal , Jun Lee
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12N9/22 , C12N15/86
CPC分类号： C12N9/1276 , C12P19/34 , C12Y207/07049
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

7. 发明申请

US20060105348A1 Compositions and methods for the detection and discrimination of nucleic acids 审中-公开
标题翻译：用于检测和鉴别核酸的组合物和方法
公开(公告)号：US20060105348A1
公开(公告)日：2006-05-18
申请号：US10987453
申请日：2004-11-15
申请人： Jun Lee , Robert Potter , Sandrine Javorschi-Miller , Jonathan Nery
发明人： Jun Lee , Robert Potter , Sandrine Javorschi-Miller , Jonathan Nery
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/686 , C12Q2563/107 , C12Q2535/125 , C12Q2525/15
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

8. 发明申请

US20070020622A1 DNA Polymerases and mutants thereof 审中-公开
标题翻译： DNA聚合酶及其突变体
公开(公告)号：US20070020622A1
公开(公告)日：2007-01-25
申请号：US10244081
申请日：2002-09-16
申请人： Jun Lee , Gary Gerard , Harini Shandilya , Katherine Griffiths , Moreland Gibbs , Peter Bergquist , Robert Potter
发明人： Jun Lee , Gary Gerard , Harini Shandilya , Katherine Griffiths , Moreland Gibbs , Peter Bergquist , Robert Potter
IPC分类号： C12Q1/68 , C07H21/04 , C12N9/22
CPC分类号： C12N9/1252 , C12N9/1276 , C12N9/14 , C12P19/34 , C12Q1/6834 , C12Q1/6869 , C12Y207/07007 , C12Y306/01 , C12Q2521/101
摘要： The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may posses both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.
摘要翻译：本发明提供具有核苷酸聚合酶活性的多肽和增强聚合酶活性的方法。本发明的多肽可以具有DNA依赖性DNA聚合酶活性和RNA依赖性DNA聚合酶活性，即逆转录酶活性。本发明的多肽可用于任何应用，包括但不限于DNA测序反应，扩增反应，cDNA合成反应以及合并的cDNA合成和扩增反应，例如RT-PCR。

9. 发明申请

US20080093125A1 Method and System for Forming a Non-Circular Borehole 审中-公开
标题翻译：形成非圆形井眼的方法和系统
公开(公告)号：US20080093125A1
公开(公告)日：2008-04-24
申请号：US11691445
申请日：2007-03-26
申请人： Jared Potter , Robert Potter , James Basler , Thomas Wideman
发明人： Jared Potter , Robert Potter , James Basler , Thomas Wideman
IPC分类号： E21B7/18
CPC分类号： E21B7/001 , F03G7/04 , F24T10/15 , F24T2010/53 , Y02E10/125
摘要： System and methods for creating shaped, non-circular boreholes in rocks especially for use with geothermal heat pump applications and for increasing wellbore support in applications such as horizontal oil and gas drilling are described. The systems and methods when applied to geothermal heat pumps create an elliptical shaped hole that is optimized for placing heat transfer tubes with a minimum of grout used. The significantly reduced cross-sectional area of the elliptical borehole also increases the overall drilling rate in rock and especially in hard rocks. In horizontal hard-rock drilling, creation of a horizontal non-circular borehole or modification of a circular borehole to a non-circular geometry is used to stabilize the borehole prior to casing insertion, and may also allow the use of lower mud pressures improving drilling rates. The system uses a non-contacting drilling system which in one embodiment uses a supersonic flame jet drilling system with a movable nozzle that swings between pivot points. In a second embodiment the elliptical shaped hole is created by an abrasive fluid or particle bearing-fluid or air jet drill that moves between pivot points. In another embodiment a non-contacting drill can use dual parallel nutating nozzles that create a pair of overlapping circular holes. The non-circular shaped hole is created by either the high temperature flame or water-particle jet or chemically active fluid jet as it removes rock material by erosion, dissolution and or thermal spalling. Modifications of circular boreholes to a generally elliptical shape can also be done using milling or jetting techniques.
摘要翻译：描述了用于在岩石中形成特殊用于地热热泵应用的成形非圆形钻孔并用于在诸如水平油气钻井的应用中增加井筒支撑的系统和方法。当应用于地热热泵时，系统和方法产生一个椭圆形孔，其优化用于放置最少使用的灌浆的传热管。椭圆孔的横截面积显着减小也增加了岩石，特别是硬岩中的总体钻井速度。在水平硬岩钻井中，在套管插入之前，使用水平非圆形钻孔或圆形钻孔修正为非圆形几何形状来稳定钻孔，并且也可允许使用较低的泥浆压力来改善钻井价格。该系统使用非接触钻井系统，其在一个实施例中使用具有在枢转点之间摆动的可移动喷嘴的超音速火焰喷射钻井系统。在第二实施例中，椭圆形孔由在枢轴点之间移动的研磨流体或颗粒轴承 - 流体或空气喷射钻头产生。在另一个实施例中，非接触钻头可以使用形成一对重叠圆形孔的双平行马蹄形喷嘴。非圆形孔通过高温火焰或水粒子喷射或化学活性流体射流产生，因为它通过侵蚀，溶解和/或热剥落来除去岩石材料。通常使用铣削或喷射技术也可以将圆形钻孔修改成大致椭圆形状。

10. 发明申请

US20070137368A1 TWO-POSITION LOCKING DEVICE FOR A SUPPORT FIXTURE 审中-公开
标题翻译：用于支撑装置的两个位置锁定装置
公开(公告)号：US20070137368A1
公开(公告)日：2007-06-21
申请号：US11562696
申请日：2006-11-22
申请人： Joseph DeVille , Robert Potter
发明人： Joseph DeVille , Robert Potter
IPC分类号： G05G11/00
CPC分类号： B65D19/44 , B65D2585/6887 , Y10T74/2025
摘要： A support fixture supports a plurality of components on a shipping rack. The support fixture includes a frame, a plurality of fingers and a locking device. Each finger is pivotally coupled to the frame for movement about a pivot between a support position and a released position. Each finger has a front end and a back end extending from substantially opposite sides of a pivot wherein the front end is presented for supporting one of the components in the support position. The fingers are arranged in a stack with a first finger being disposed at an end of the stack. Each of the fingers has a boss that extends outwardly therefrom to hold an adjacent finger in the support position. The locking device has a pin that is axially movable between an extended position disposed along the back end of the first finger to hold the first finger in the support position and a retracted position spaced apart spaced apart from the first finger to allow pivotal movement of the first finger between the support and released positions. The locking device also has a button operatively coupled to the pin to cause alternating actuation of the pin between the extended and retracted positions with each push of the button toward the pin.
摘要翻译：支撑夹具支撑运输架上的多个部件。支撑夹具包括框架，多个指状物和锁定装置。每个手指枢转地联接到框架，用于围绕支撑位置和释放位置之间的枢轴运动。每个手指具有从枢轴的基本相对的侧面延伸的前端和后端，其中呈现前端以支撑支撑位置中的一个部件。手指被布置成堆叠，其中第一指状物设置在堆叠的端部。每个指状物具有从其向外延伸的凸起，以将相邻的手指保持在支撑位置。锁定装置具有销，该销可在沿着第一手指的后端设置的延伸位置之间轴向移动以将第一手指保持在支撑位置，并且与第一手指间隔开的缩回位置允许枢轴运动第一手指在支撑位置和释放位置之间。锁定装置还具有可操作地联接到销的按钮，以使销在推动位置和缩回位置之间的交替致动，同时按钮朝向销。

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